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[1]伍捷進(jìn),吳啟富,接紅宇,等.健骨顆粒沖劑對膝骨關(guān)節(jié)炎大鼠血清和關(guān)節(jié)液中腫瘤壞死 因子-α及基質(zhì)金屬蛋白酶-3的影響[J].中醫(yī)正骨,2012,24(05):20-22.
　WU Jie-jin*,WU Qi-fu,JIE Hong-yu,et al.Study on the effect of JIANGU INSTANT GRANULES on tumor necrosis factor-α and matrix metalloproteinase-3 in blood serum and synovial fluid for rats with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(05):20-22.
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健骨顆粒沖劑對膝骨關(guān)節(jié)炎大鼠血清和關(guān)節(jié)液中腫瘤壞死 因子-α及基質(zhì)金屬蛋白酶-3的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年05期
頁碼:
20-22
欄目:
基礎(chǔ)研究
出版日期:
2012-05-20

文章信息/Info

Title:
Study on the effect of JIANGU INSTANT GRANULES on tumor necrosis factor-α and matrix metalloproteinase-3 in blood serum and synovial fluid for rats with knee osteoarthritis
作者:
伍捷進(jìn)1; 吳啟富1; 接紅宇1; 戚晶敏1; 黃綏心2; 王繁盛1
1.南方醫(yī)科大學(xué)中醫(yī)藥學(xué)院,廣東 廣州 510515;
2.浙江省新華醫(yī)院,浙江 杭州 310005
Author(s):
WU Jie-jin*; WU Qi-fu; JIE Hong-yu; QI Jing-min; HUANG Sui-xin; WANG Fan-sheng.
*Traditional Chinese Medicical College of Southern Medical University,Guangzhou 510515,Guangdong,China
關(guān)鍵詞:
骨關(guān)節(jié)炎; 膝 腫瘤壞死因子-α 基質(zhì)金屬蛋白酶-3 健骨顆粒沖劑 動物實驗
Keywords:
Osteoarthritis; knee;  Tumor necrosis factor-alpha;  Matrix metalloproteinase 3;  JIANGU INSTANT GRANULES;  Animal experimentation
摘要:
目的:觀察健骨顆粒沖劑對膝骨關(guān)節(jié)炎大鼠血清和關(guān)節(jié)液中腫瘤壞死因子-α及基質(zhì)金屬蛋白酶-3的影響,探討健骨顆粒沖劑治療膝骨關(guān)節(jié)炎的作用機(jī)制。方法:將48只Wistar大鼠適應(yīng)性喂養(yǎng)1周后,隨機(jī)抽取40只分為正常組、模型組、雙醋瑞因組及健骨顆粒沖劑組,每組10只。分組結(jié)束后,將模型組、雙醋瑞因組及健骨顆粒沖劑組大鼠制作膝骨關(guān)節(jié)炎動物模型。造模成功后,雙醋瑞因組大鼠按0.009 g·kg-1以雙醋瑞因溶液灌胃,健骨顆粒沖劑組大鼠按2.7 g·kg-1以健骨顆粒沖劑溶液灌胃,正常組和模型組給予相應(yīng)劑量的生理鹽水,每日1次,連續(xù)給藥4周。藥物干預(yù)結(jié)束后,采用酶聯(lián)免疫吸附法檢測各組大鼠血清和關(guān)節(jié)液中腫瘤壞死因子-α及基質(zhì)金屬蛋白酶-3的含量。結(jié)果:①血清腫瘤壞死因子-α含量。各組間含量比較,差異有統(tǒng)計學(xué)意義(F=10.056,P=0.008)。組間兩兩比較,模型組高于正常組、雙醋瑞因組及健骨顆粒沖劑組(P=0.000,P=0.000,P=0.000); 其余各組間比較,差異均無統(tǒng)計學(xué)意義。②血清基質(zhì)金屬蛋白酶-3含量。各組間含量比較,差異有統(tǒng)計學(xué)意義(F=8.874,P=0.013)。組間兩兩比較,模型組高于正常組、雙醋瑞因組及健骨顆粒沖劑組(P=0.000,P=0.000,P=0.000); 其余各組間比較,差異均無統(tǒng)計學(xué)意義。③關(guān)節(jié)液腫瘤壞死因子-α含量。各組間含量比較,差異有統(tǒng)計學(xué)意義(F=10.182,P=0.006)。組間兩兩比較,模型組高于正常組、雙醋瑞因組及健骨顆粒沖劑組(P=0.000,P=0.000,P=0.000); 其余各組間比較,差異均無統(tǒng)計學(xué)意義。④關(guān)節(jié)液基質(zhì)金屬蛋白酶-3含量。各組間含量比較,差異有統(tǒng)計學(xué)意義(F=8.476,P=0.017)。組間兩兩比較,模型組高于正常組、雙醋瑞因組及健骨顆粒沖劑組(P=0.000,P=0.000,P=0.000); 其余各組間比較,差異均無統(tǒng)計學(xué)意義。結(jié)論:健骨顆粒沖劑能降低膝骨關(guān)節(jié)炎大鼠血清及關(guān)節(jié)液中腫瘤壞死因子-α及基質(zhì)金屬蛋白酶-3的含量,從而減輕其對關(guān)節(jié)軟骨基質(zhì)的損害,直接或間接改善軟骨基質(zhì)代謝,促進(jìn)軟骨修復(fù)。
Abstract:
Objective:To observe the effect of JIANGU INSTANT GRANULES on tumor necrosis factor-α(TNF-α)and matrix metalloproteinase-3(MMP-3)in blood serum and synovial fluid for rats with knee osteoarthritis(KOA),and to explore the mechanism of JIANGU INSTANT GRANULES in the treatment of KOA.Methods:After 1 week adaptive feeding,40 rats were selected from 48 Wistar rats and randomly divided into normal group,model group,diacerein group and JIANGU INSTANT GRANULES group,10 cases in each group.After grouping,rats in model group,diacerein group and JIANGU INSTANT GRANULES group were built KOA animal models.After successful modeling,rats in diacerein group were administrated by gavage with diacerein solution(0.009 g·kg-1),rats in JIANGU INSTANT GRANULES group were administrated by gavage with JIANGU INSTANT GRANULES solution(2.7 g·kg-1),while rats in normal group and model group were administrated by gavage with corresponding doses of normal saline,once a day for 4 consecutive weeks.After drug treatment,TNF-α and MMP-3 contents in blood serum and synovial fluid of all the rats were measured through enzyme linked immunosorbent assay.Results:①TNF-α content in blood serum:there was statistical difference in the contents among the 4 groups(F=10.056,P=0.008).The content of model group was higher than that of normal group,diacerein group and JIANGU INSTANT GRANULES group(P=0.000,P=0.000,P=0.000); while there was no statistical difference in contents between any other groups.②MMP-3 content in blood serum:there was statistical difference in the contents among the 4 groups(F=8.874,P=0.013).The content of model group was higher than that of normal group,diacerein group and JIANGU INSTANT GRANULES group(P=0.000,P=0.000,P=0.000); while there was no statistical difference in contents between any other groups.③TNF-α content in synovial fluid:there was statistical difference in the contents among the 4 groups(F=10.182,P=0.006).The content of model group was higher than that of normal group,diacerein group and JIANGU INSTANT GRANULES group(P=0.000,P=0.000,P=0.000); while there was no statistical difference in contents between any other groups.④MMP-3 content in synovial fluid:there was statistical difference in the contents among the 4 groups(F=8.476,P=0.017).The content of model group was higher than that of normal group,diacerein group and JIANGU INSTANT GRANULES group(P=0.000,P=0.000,P=0.000); while there was no statistical difference in contents between any other groups.Conclusion:JIANGU INSTANT GRANULES can directly or indirectly improve cartilage matrix metabolism to promote cartilage repair through lowering TNF-α and MMP-3 contents in blood serum and synovial fluid for KOA rats to relieve their damage on articular cartilage matrix.

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通訊作者:吳啟富 E-mail:btdy168@163.com
更新日期/Last Update: 2012-05-20