84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]湯劍斌,圣小平,樊天佑.電針治療對膝骨關節(jié)炎兔膝關節(jié)軟骨細胞凋亡的影響[J].中醫(yī)正骨,2012,24(04):12-15.
　TANG Jian-bin*,SHENG Xiao-ping,FAN Tian-you.*.Study on the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(04):12-15.
點擊復制

電針治療對膝骨關節(jié)炎兔膝關節(jié)軟骨細胞凋亡的影響()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年04期
頁碼:
12-15
欄目:
基礎研究
出版日期:
2012-04-30

文章信息/Info

Title:
Study on the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis
作者:
湯劍斌1; 圣小平2; 樊天佑2
1.上海市浦東新區(qū)光明中醫(yī)醫(yī)院,上海 201399;
2.上海市中醫(yī)醫(yī)院,上海 200071
Author(s):
TANG Jian-bin*; SHENG Xiao-ping; FAN Tian-you.*
Guangming Hospital of Traditional Chinese Medicine of Pudong New Area in Shanghai City,Shanghai 201399,China
關鍵詞:
骨關節(jié)炎; 膝 電針 軟骨細胞 細胞凋亡
Keywords:
Osteoarthritis; knee;  Electroacupuncture;  Chondrocytes;  Apoptosis
摘要:
目的:觀察電針治療對膝骨關節(jié)炎兔膝關節(jié)軟骨細胞凋亡的影響,探討電針治療膝骨關節(jié)炎的作用機制。方法:將30只健康新西蘭大白兔隨機分為正常對照組、模型對照組和電針治療組,每組10只。采用Hulth法對模型對照組及電針治療組實驗兔左膝關節(jié)進行造模,造模后第5周開始對電針治療組實驗兔進行電針治療,正常對照組、模型對照組同期正常飼養(yǎng),不作任何治療。治療4周后同時處死3組實驗兔,取左膝關節(jié)股骨端制成切片,觀察各組實驗兔軟骨組織形態(tài)及軟骨細胞凋亡情況。結果:①軟骨組織形態(tài)。正常對照組軟骨表層光滑、平整,4層結構清晰,軟骨細胞分布均勻、無簇聚,潮線完整; 模型對照組關節(jié)軟骨厚度減小,表面毛糙,可見明顯纖維化、裂隙、軟骨細胞簇聚,潮線斷裂; 電針治療組關節(jié)軟骨厚度減小,表面不光滑,少見纖維化、裂隙、軟骨細胞簇聚,潮線斷裂。②軟骨細胞凋亡情況。3組實驗兔軟骨細胞凋亡率比較,差異有統(tǒng)計學意義(F=126.753,P=0.000); 進一步兩兩比較,模型對照組軟骨細胞凋亡率大于正常對照組和電針治療組(LSD-t=3.769,P=0.000; LSD-t=7.000,P=0.000),電針治療組軟骨細胞凋亡率大于正常對照組(LSD-t=10.769,P=0.000)。結論:電針治療可以通過抑制軟骨細胞凋亡,減輕膝骨關節(jié)炎軟骨的損傷,這可能是電針治療膝骨關節(jié)炎的機制之一。
Abstract:
Objective:To observe the effect of electroacupuncture on knee joint chondrocyte apoptosis in rabbits with knee osteoarthritis(KOA),and to explore the mechanisms of electroacupuncture on KOA.Methods:Thirty healthy New Zealand white rabbits were randomly divided into 3 groups as normal control group,model control group and electroacupuncture group,10 cases in each group.Left knee joints of experimental rabbits in model control group and electroacupuncture group were all built models through Hulth method.At the 5th week after modeling,cases in electroacupuncture group were administrated with electroacupuncture,while the others in the normal control group and model control group were administrated with normal feeding without treatment.After 4 weeks of treatment,all of the experimental rabbits were executed for making slices of left femoral condyle which were observed on the morphous of cartilaginous tissue and chondrocyte apoptosis situation.Results:①The morphous of cartilaginous tissue:the situations as smooth cartilage surface,clearness in four-layer structure,evenly distribution of chondrocytes without clustering and complete tidemark were found in normal control group; the situations as decrease in articular cartilage thickness,rough surface,significant fibrosis and fissures,clustered chondrocytes and fractured tidemark were found in model control group; and the situations as decrease in articular cartilage thickness,unsmooth surface,rare fibrosis and fissures,clustered chondrocytes and fractured tidemark were found in electroacupuncture group.②Chondrocyte apoptosis situation:there were statistical difference in chondrocyte apoptosis rate of experimental rabbits among the 3 groups(F=126.753,P=0.000).Chondrocyte apoptosis rate of model control group was higher than that of normal control group and electroacupuncture group respectively(LSD-t=3.769,P=0.000; LSD-t=7.000,P=0.000),and the chondrocyte apoptosis rate of electroacupuncture group was higher than that of normal control group(LSD-t=10.769,P=0.000).Conclusion:Electroacupuncture can relieve articular cartilage injuries of KOA through inhibiting chondrocyte apoptosis,which may be one of the mechanisms of electroacupuncture on KOA.

參考文獻/References:

[1] Jubb RW,Tukmachi ES,Jones PW,et al.A blinded randomised trial of acupuncture(manual and electroacupuncture)compared with a non-penetrating sham for the symptoms of osteoarthritis of the knee[J].Acupunct Med,2008,26(2):69-78.
[2] Itoh K,Hirota S,Katsumi Y,et al.A pilot study on using acupuncture and transcutaneous electrical nerve stimulation(TENS)to treat knee osteoarthritis(OA)[J].Chin Med,2008,3:2.
[3] Witt CM,Jena S,Brinkhaus B,et al.Acupuncture in patients with osteoarthritis of the knee or hip:a randomized,controlled trial with an additional nonrandomized arm[J].Arthritis Rheum,2006,54(11):3485-3493.
[4] Vas J,Méndez C,Perea-Milla E,et al.Acupuncture as a complementary therapy to the pharmacological treatment of osteoarthritis of the knee:randomised controlled trial[J].BMJ,2004,329(7476):1216.
[5] Berman BM,Lao L,Langenberg P,et al.Effectiveness of acupuncture as adjunctive therapy in osteoarthritis of the knee:a randomized,controlled trial[J].Ann Intern Med,2004,141(12):901-910.
[6] Hulth A,Lindberg L,Telhag H.Experimental osteoarthritis in rabbits.Preliminary report[J].Acta Orthop Scand,1970,41(5):522-530.
[7] Moskwitz RW,Altman RD,Buckwalter JA,et al.Osteoarthritis:diagnosis and medical/surgical management[M].3rd ed.Philadelphia:Lippincott Williams&Wilkins,2001:1.
[8] 王瑞輝,楊介賓.電針對佐劑性關節(jié)炎大鼠IL-1和IL-6活性的影響[J].陜西中醫(yī)學院學報,2001,24(4):43-45.
[9] 丘德興.不同針灸法對膝骨性關節(jié)炎療效對比的實驗與臨床研究[D].成都:成都中醫(yī)藥大學,2004.
[10] 鄭秀存.針刺、電針和溫針治療對硒鼓性關節(jié)炎(KOA)軟骨基質代謝調節(jié)作用的實驗研究[D].成都:成都中醫(yī)藥大學,2005.
[11] 翁文水.針灸治療家兔膝骨關節(jié)骨性關節(jié)炎的實驗研究[D].福州:福建中醫(yī)學院,2004.
[12] Blanco FJ,Guitian R,Vázquez-Martul E,et al.Osteoarthritis chondrocytes die by apoptosis.A possible pathway for osteoarthritis pathology[J].Arthritis Rheum,1998,41(2):284-289.
[13] Aigner T,Hemmel M,Neureiter D,et al.Apoptotic cell death is not a widespread phenomenon in normal aging and osteoarthritis human articular knee cartilage:a study of proliferation,programmed cell death(apoptosis),and viability of chondrocytes in normal and osteoarthritic human knee cartilage[J].Arthritis Rheum,2001,44(6):1304-1312.
[14] Watrin-Pinzano A,Etienne S,Grossin L,et al.Increased apoptosis in rat osteoarthritic cartilage corresponds to degenerative chondral lesions and concomitant expression of caspase-3[J].Biorheology,2006,43(3-4):403-412.
[15] Kim HA,Lee YJ,Seong SC,et al.Apoptotic chondrocyte death in human osteoarthritis[J].J Rheumatol,2000,27(2):455-462.
[16] Hashimoto S,Takahashi K,Amiel D,et al.Chondrocyte apoptosis and nitric oxide production during experimentally induced osteoarthritis[J].Arthritis Rheum,1998,41(7):1266-1274.
[17] 吳明霞,李西海,吳廣文,等.電針后血清對腫瘤壞死因子α誘導軟骨細胞凋亡的影響[J].中國組織工程研究與臨床康復,2011,15(46):8551-8555.

相似文獻/References:

[1]孟維娜,明立功,王新德,等.關節(jié)鏡下清理聯(lián)合腓骨近1/3段截骨治療膝骨關節(jié)炎[J].中醫(yī)正骨,2015,27(11):40.
[2]明立功,孟維娜,王新德,等.腓骨近端截骨治療內側間室膝骨關節(jié)炎的近期療效觀察[J].中醫(yī)正骨,2015,27(10):25.
[3]張杰,王人彥,張玉柱.膝骨關節(jié)炎的治療進展[J].中醫(yī)正骨,2015,27(10):68.
[4]梁朝,蔡靜怡,閆立,等.針刀療法改善膝骨關節(jié)炎早期疼痛癥狀的療效評價[J].中醫(yī)正骨,2015,27(09):9.
　LIANG Zhao,CAI Jingyi,YAN Li,et al.Evaluation of the curative effect of needle-knife therapy for relieving knee pain in patients with early knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):9.
[5]王建武,黨建軍,李強,等.四聯(lián)療法治療膝骨關節(jié)炎[J].中醫(yī)正骨,2015,27(08):44.
[6]劉紅娟,郭會利,郭樹農.云克聯(lián)合中藥治療膝骨關節(jié)炎的護理[J].中醫(yī)正骨,2015,27(08):75.
[7]陳衛(wèi)衡.探索建立系統(tǒng)的膝骨關節(jié)炎中醫(yī)臨床科研范式 和理論體系[J].中醫(yī)正骨,2015,27(07):1.
[8]鄭春松,葉蕻芝,李西海,等.透骨消痛膠囊中補腎柔肝藥和活血祛風藥治療 骨關節(jié)炎作用方式的計算機模擬比較[J].中醫(yī)正骨,2015,27(07):6.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Comparison of the mode of action of Bushen Rougan(補腎柔肝)drugs versus Huoxue Qufeng(活血祛風)drugs contained in Tougu Xiaotong Jiaonang(透骨消痛膠囊)for the treatment of osteoarthritis:A computer simulation study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):6.
[9]帥波,沈霖,楊艷萍,等.加味青娥丸治療膝骨關節(jié)炎的作用機制研究[J].中醫(yī)正骨,2015,27(07):15.
　SHUAI Bo,SHEN Lin,YANG Yanping,et al.Study on the mechanism of action of Jiawei Qing'e Wan(加味青娥丸)for the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):15.
[10]梅其杰,袁長深,段戡,等.壯藥骨痹方燙熨聯(lián)合運動療法治療膝骨關節(jié)炎的臨床研究[J].中醫(yī)正骨,2015,27(07):27.
　MEI Qijie,YUAN Changshen,DUAN Kan,et al.Clinical study of the curative effect of hot compressing and rubbing with packet of Gubi Fang(骨痹方)combined with exercise therapy in the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(04):27.

備注/Memo

備注/Memo:
2011-08-17收稿 2012-02-14修回
更新日期/Last Update: 2012-04-30