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[1]戴斌,沈海濱,李道龍,等.甲基強(qiáng)的松龍聯(lián)合益腎通痹湯 對大鼠壓迫性神經(jīng)根損傷的保護(hù)作用[J].中醫(yī)正骨,2012,24(03):9-14.
　DAI Bin*,SHEN Hai-bin,LI Dao-long,et al.The protective effect of methylprednisolone combined with YISHEN TONGBI decoction on nerve root compressive injury in rat[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(03):9-14.
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甲基強(qiáng)的松龍聯(lián)合益腎通痹湯 對大鼠壓迫性神經(jīng)根損傷的保護(hù)作用()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年03期
頁碼:
9-14
欄目:
基礎(chǔ)研究
出版日期:
2012-03-01

文章信息/Info

Title:
The protective effect of methylprednisolone combined with YISHEN TONGBI decoction on nerve root compressive injury in rat
作者:
戴斌1; 沈海濱1; 李道龍1; 王金榮1; 周克中1; 許建安2; 陳剛2; 李志偉2
1.江蘇省濱海縣人民醫(yī)院,江蘇 濱海 224500;
2.江蘇省中醫(yī)院,江蘇 南京 210019
Author(s):
DAI Bin*; SHEN Hai-bin; LI Dao-long; WANG Jin-rong; ZHOU Ke-zhong; XU Jian-an; CHEN Gang; LI Zhi-wei.*
The People's Hospital of Binhai county,Binhai 224500,Jiangsu,China
關(guān)鍵詞:
神經(jīng)根病 益腎通痹湯 甲潑尼龍 動物實驗
Keywords:
Radiculopathy;  YISHEN TONGBI DECOCTION;  Methylprednisolone;  Animal experimentation
摘要:
目的:探討甲基強(qiáng)的松龍聯(lián)合益腎通痹湯對大鼠壓迫性神經(jīng)根損傷的保護(hù)作用。方法:將90只SD大鼠隨機(jī)分為5組,即空白 組、模型組、甲基強(qiáng)的松龍組、中藥組、甲基強(qiáng)的松龍+中藥組,每組18只。采用王擁軍等建立的腰神經(jīng)根壓迫造模法對模型組 、甲基強(qiáng)的松龍組、中藥組和甲基強(qiáng)的松龍+中藥組大鼠進(jìn)行造模,空白組大鼠切開背部后立即縫合。造模成功后,甲基強(qiáng)的松龍 組以小劑量甲基強(qiáng)的松龍腹腔注射,中藥組以益腎通痹湯灌胃,甲基強(qiáng)的松龍+中藥組以小劑量甲基強(qiáng)的松龍腹腔注射并以益腎通 痹湯灌胃; 空白組和模型組僅以相同劑量的蒸餾水灌胃。分別于造模后第7天、第14天及第28天對各組大鼠的行為學(xué)特征和神經(jīng) 根損傷區(qū)變化進(jìn)行觀察。結(jié)果:①行為學(xué)特征:7 d時甲基強(qiáng)的松龍、益腎通痹湯單獨使用均能降低大鼠行為學(xué)特征評分,且二者 聯(lián)合使用具有協(xié)同效應(yīng)(甲基強(qiáng)的松龍:F=61.309,P=0.000; 益腎通痹湯:F=98.078,P=0.000; 聯(lián)合應(yīng)用:F=39.654,P=0.001); 14 d時甲基強(qiáng)的松龍、益腎通痹湯單獨使用均能降低大鼠行為學(xué)特征評分,且二者聯(lián)合使用具有協(xié)同效應(yīng)(甲基強(qiáng)的松 龍:F=156.902,P=0.000; 益腎通痹湯:F=196.267,P=0.000; 聯(lián)合應(yīng)用:F=29.512,P=0.002); 28 d時甲基強(qiáng)的松龍、益腎通痹湯 單獨使用均能降低大鼠行為學(xué)特征評分,且二者聯(lián)合使用具有協(xié)同效應(yīng)(甲基強(qiáng)的松龍:F=96.098,P=0.000; 益腎通痹 湯:F=78.204,P=0.000; 聯(lián)合應(yīng)用:F=19.090,P=0.001)。②正常神經(jīng)元:7 d時各組正常神經(jīng)元數(shù)量比較,差異有統(tǒng)計學(xué)意義(F= 42.230,P=0.001); 進(jìn)一步兩兩比較,除模型組與中藥組、甲基強(qiáng)的松龍組與甲基強(qiáng)的松龍+中藥組比較差異無統(tǒng)計學(xué)意義外 (P=0.087; P=0.069),其余各組間比較,差異均有統(tǒng)計學(xué)意義。14 d時各組正常神經(jīng)元數(shù)量比較,差異有統(tǒng)計學(xué)意義 (F=23.098,P=0.002); 進(jìn)一步兩兩比較,除模型組與甲基強(qiáng)的松龍組比較差異無統(tǒng)計學(xué)意義外(P=0.067),其余各組間比較,差異 均有統(tǒng)計學(xué)意義。28 d時各組正常神經(jīng)元數(shù)量比較,差異有統(tǒng)計學(xué)意義(F=34.543,P=0.001); 進(jìn)一步兩兩比較,除模型組與甲基 強(qiáng)的松龍組比較差異無統(tǒng)計學(xué)意義外(P=0.098),其余各組間比較,差異均有統(tǒng)計學(xué)意義。③變性神經(jīng)元:7 d時甲基強(qiáng)的松龍、益 腎通痹湯單獨使用均能減少變性神經(jīng)元數(shù)量,且二者聯(lián)合使用具有協(xié)同效應(yīng)(甲基強(qiáng)的松龍:F=154.521,P=0.000; 益腎通痹 湯:F=102.721,P=0.000; 聯(lián)合應(yīng)用:F=19.512,P=0.002); 14 d時甲基強(qiáng)的松龍、益腎通痹湯單獨使用均能減少變性神經(jīng)元數(shù)量, 且二者聯(lián)合使用具有協(xié)同效應(yīng)(甲基強(qiáng)的松龍:F=54.912,P=0.000; 益腎通痹湯:F=60.451,P=0.001; 聯(lián)合應(yīng) 用:F=12.720,P=0.002); 28 d時甲基強(qiáng)的松龍、益腎通痹湯單獨使用均能減少變性神經(jīng)元數(shù)量,且二者聯(lián)合使用具有協(xié)同效應(yīng)( 甲基強(qiáng)的松龍:F=67.912,P=0.001; 益腎通痹湯:F=53.109,P=0.001; 聯(lián)合應(yīng)用:F=9.560,P=0.002)。結(jié)論:甲基強(qiáng)的松龍聯(lián)合益 腎通痹湯能夠減輕機(jī)械壓迫性神經(jīng)根損傷所導(dǎo)致的神經(jīng)功能及組織形態(tài)病理學(xué)改變,促進(jìn)損傷神經(jīng)元修復(fù)以及正常神經(jīng)元再生, 具有改善神經(jīng)功能、保護(hù)神經(jīng)根的作用。
Abstract:
Objective:To explore the protective effect of methylprednisolone(MP)combined with YISHEN TONGBI decoction on nerve root compressive injury in rat.Methods:Ninety Sprague-Dawley rats were randomly divided into 5 groups as blank group,model group,MP group,traditional Chinese medicine(TCM)group and MP combined with TCM group,18 cases in each group.Rats in model group,MP group,TCM group and MP combined with TCM group were built models through the compressive molding method of lumbar nerve root formulated by Wang Yong-jun,while the others in the blank group were processed with immediately suture after incision at the back.After successful modeling,rats in MP group were administrated with intraperitoneal injection of low doses of MP,cases in TCM group were administrated with gastric perfusion of YISHEN TONGBI decoction,cases in MP combined with TCM group were administrated with intraperitoneal injection of low doses of MP combined with gastric perfusion of YISHEN TONGBI decoction,while the others in blank group and model group were administrated with gastric perfusion of same doses of distilled water only.The behavioural features and changes in nerve root injury zones of the rats were observed at the 7th,the 14th and the 28th day after modeling respectively.Results:①Behavioural features:at the 7th day after modeling,the scores of behavioural features of rats were lowered down through the separately using of MP and YISHEN TONGBI decoction,and synergistic effect was found after combined application of above methods(MP:F=61.309,P=0.000; YISHEN TONGBI decoction:F=98.078,P=0.000; combined application:F=39.654,P=0.001); and above situations were also applied to those of rats at the 14th day after modeling(F=156.902,P=0.000; YISHEN TONGBI decoction:F=196.267,P=0.000; combined application:F=29.512,P=0.002)and at the 28th day after modeling (F=96.098,P=0.000; YISHEN TONGBI decoction:F=78.204,P=0.000; combined application:F=19.090,P=0.001) respectively.②The number of normal neurons:at the 7th day after modeling,there was statistical difference in the number of normal neurons among the 5 groups(F=42.230,P=0.001); there was statistical difference between any other couple groups except between model group and TCM group(P=0.087),between MP group and MP combined with TCM group(P=0.069)according to the further paired comparison approach.At the 14th day after modeling,there was statistical difference in the number of normal neurons among the 5 groups(F=23.098,P=0.002); there was statistical difference between any other couple groups except between model group and MP group(P=0.067) according to the further paired comparison approach.At the 28th day after modeling,there was statistical difference in the number of normal neurons among the 5 groups(F=34.543,P=0.001); there was statistical difference between any other couple groups except between model group and MP group(P=0.098)according to the further paired comparison approach.③The number of degenerated neurons:at the 7th day after modeling,the number of degenerated neurons were decreased through the separately using of MP and YISHEN TONGBI decoction,and synergistic effect was found after combined application of above methods(MP:F=154.521,P=0.000; YISHEN TONGBI decoction:F=102.721,P=0.000; combined application:F=19.512,P=0.002); and above situations were also applied to those of rats at the 14th day after modeling(MP:F=54.912,P=0.000; YISHEN TONGBI decoction:F=60.451,P=0.001; combined application:F=12.720,P=0.002)and at the 28th day after modeling(MP:F=67.912,P=0.001; YISHEN TONGBI decoction:F=53.109,P=0.001; combined application:F=9.560,P=0.002)respectively.Conclusion:The application of MP combined with YISHEN TONGBI decoction has the effects of improving neural functions and protecting nerve root through relieving the neurological dysfunction and pathological changes caused by nerve root compressive injury and also promoting the reparation of the injured neurons and rebirth of the normal neurons.

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備注/Memo

備注/Memo:
2011-02-25收稿 2011-06-20修回
通迅作者:戴斌 E-mail:[email protected]
更新日期/Last Update: 2012-03-30