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[1]邱明山,陳進(jìn)春,徐振興,等.獨(dú)活對大鼠膠原誘導(dǎo)性關(guān)節(jié)炎的治療作用[J].中醫(yī)正骨,2012,24(02):6-8.
　QIU Ming-shan*,CHEN Jin-chun,ZHANG Yu-yan,et al.Effect of Heracleum on collagen induced arthritis in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2012,24(02):6-8.
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獨(dú)活對大鼠膠原誘導(dǎo)性關(guān)節(jié)炎的治療作用()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第24卷
期數(shù):
2012年02期
頁碼:
6-8
欄目:
基礎(chǔ)研究
出版日期:
2012-02-25

文章信息/Info

Title:
Effect of Heracleum on collagen induced arthritis in rats
作者:
邱明山; 陳進(jìn)春; 徐振興; 張怡燕; 蔣玉芬; 彭海聰
福建省廈門市中醫(yī)院,福建 廈門 361009
Author(s):
QIU Ming-shan*; CHEN Jin-chun; ZHANG Yu- yan; ZHANG Yi-yan; JIANG Yu-fen; PENG Hai-cong.*
Affiliated Xiamen TCM Hospital of Fujian TCM University,Xiamen 361001,Fujian,China
關(guān)鍵詞:
獨(dú)活 關(guān)節(jié)炎; 類風(fēng)濕 動物實驗 大鼠
Keywords:
Heracleum;  Collagen induced arthritis
摘要:
目的:觀察不同劑量獨(dú)活對膠原誘導(dǎo)性關(guān)節(jié)炎的治療作用。方法:選擇牛Ⅱ型膠原為免疫原,建立膠原誘導(dǎo)性關(guān)節(jié)炎模型, 選取30只造模成功的大鼠隨機(jī)分為5組:獨(dú)活組[按順序再分為低劑量(1.5 g·kg-1)組、中劑量(4.5 g·kg- 1)組、大劑量(15 g·kg-1)組]和甲胺喋呤(B)組、生理鹽水(C)組。采用關(guān)節(jié)炎指數(shù)評分對大鼠關(guān)節(jié)炎程度 進(jìn)行評分; 采用酶聯(lián)免疫吸附法測定各組大鼠血清基質(zhì)金屬蛋白酶-3、血管內(nèi)皮生長因子,并評估可能的肝腎及胃黏膜副反應(yīng)。 結(jié)果:AI評分比較,中、小劑量獨(dú)活組與生理鹽水組差異無統(tǒng)計學(xué)意義; 大劑量獨(dú)活組小于生理鹽水組(P<0.05),獨(dú)活各劑量 組差異無統(tǒng)計學(xué)意義; 甲胺喋呤組均小于生理鹽水組、小、中劑量獨(dú)活組(分別P<0.01、P<0.05、P<0.05),而與大劑 量獨(dú)活組比較差異無統(tǒng)計學(xué)意義。血清血管內(nèi)皮生長因子比較,獨(dú)活各劑量組差異無統(tǒng)計學(xué)意義,但均顯著低于生理鹽水組(按大 、中、小分別P<0.01、P<0.05、P<0.05); 甲胺喋呤組顯著低于生理鹽水組及小劑量獨(dú)活組(分別P<0.01、 P<0.05),但與中、大劑量獨(dú)活組比較差異無統(tǒng)計學(xué)意義。血清基質(zhì)金屬蛋白酶-3比較,各獨(dú)活劑量組比較差異無統(tǒng)計學(xué)意義; 獨(dú)活小劑量組與生理鹽水組差異無統(tǒng)計學(xué)意義; 獨(dú)活中、大劑量組均低于生理鹽水組(均P<0.05); 甲胺喋呤組低于生理鹽水 組及小劑量獨(dú)活組(均P<0.01),但與中、大劑量獨(dú)活組比較差異無統(tǒng)計學(xué)意義。各組肝功、腎功、血常規(guī)及胃病理檢查均未 見明顯異常。結(jié)論:①獨(dú)活大劑量能減輕膠原誘導(dǎo)性關(guān)節(jié)炎炎癥,而中低劑量則無此作用; ②獨(dú)活中大劑量能明顯降低血清血管 內(nèi)皮生長因子、基質(zhì)金屬蛋白酶-3水平; ③獨(dú)活大劑量在短期動物實驗中無肝腎功能、血液、胃組織損害。
Abstract:
Objective:To observe the therapeutic effectiveness of different doses Heracleum on collagen induced arthritis(CIA)in rats.Methods:CIA rat model was established by subcutaneouly injecting bovine nasal cartilage II collagen.30 CIA Rats were randomly divided into three Heracleum(low,medium and high dose,A1 and A2 and A3) treated groups,MTX(Methotrexate,B)treated group,and control(C)group.The arthritic index(AI)of all rats were compared separately,the serum level of MMP-3,VEGF,AST,ALT,BUN,Cr were assayed,and the histology of gastric mucosal were examination.Result:In AI score,there was no difference between groupA2,groupA1 and groupC; that in groupA3 were less than GroupB(P<0.05,P<0.01),there was no statistical difference in Heracleum groups.In the serum level of VEGF,all the group A1 and A2 and A3 and B were less than the groupC (P<0.05,P<0.05,P<0.01,P<0.01),but the differences of groupA2 and groupA3 and groupB were not statistically significant.In the serum level of MMP-3,all the groupA2 and A3 and B were less than the groupC (P<0.05,P<0.05,P<0.01),but the differences of groupA2 and groupA3 and groupB were not statistically significant.In the serum level of AST,ALT,BUN,Cr and the histology of gastric mucosal,all groups showed no obvious abnormalities.Conclusion:①High dose of Heracleum can reduce the inflammation of CIA rats,while the low dose does not have this effect.②Medium and high dose of Heracleum can significantly reduce the serum level of VEGF and MMP-3.③For High dose of Heracleum, there are no damage of hepatic and renal function,blood and stomach in the short-term animal studies.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
2011-07-21收稿 2011-10-08修回
基金項目:福建省廈門市衛(wèi)生局青年創(chuàng)新課題基金資助項目[廈衛(wèi)科教中醫(yī)(2007)479號]
通訊作者:陳進(jìn)春 E-mail:[email protected]
更新日期/Last Update: 2012-02-25