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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:

第23卷

期數(shù):

2011年12期

頁(yè)碼:

18-21

欄目:

基礎(chǔ)研究

出版日期:

2011-12-31

文章信息/Info

Title:

Study on the function mechanism of XIAOZHONG ZHITONG capsule on acute soft tissue injury in rabbits

作者:

邢花妮¹; 隋秀麗¹; 宋文靜¹; 牟東珍²

1.山東省文登整骨醫(yī)院,山東 文登 264400; 2.濰坊醫(yī)學(xué)院,山東 濰坊 261053

Author(s):

XING Hua-ni^*; SUI Xiu-li; SONG Wen-jing; et al.^*

Orthopedics Hospital of Wendeng City,Wendeng 264400,Shandong,China

關(guān)鍵詞:

軟組織損傷 消腫止痛膠囊 白細(xì)胞介素-1β 白細(xì)胞介素-6 血液黏度 動(dòng)物實(shí)驗(yàn)

Keywords:

Soft tissue injuries;  XIAOZHONG ZHITONG capsule;  Interleukin-1beta;  Interleukin-6;  Blood viscosity;  Animal experimentation

摘要:

目的:探討消腫止痛膠囊對(duì)急性軟組織損傷的治療作用及可能的作用機(jī)制。方法:將30只健康新西蘭兔隨機(jī)分為正常組、模型組、治療組,每組10只。除正常組外,其余2組建立兔急性損傷性血腫模型。正常組及模型組按4 mL·kg^-1體質(zhì)量以生理鹽水灌胃,治療組按4 mL·kg^-1體質(zhì)量以20 mg·mL^-1消腫止痛膠囊混懸液灌胃。造模后第5天肉眼觀察傷肢損傷處的變化并參照馮芳軍等的標(biāo)準(zhǔn)進(jìn)行評(píng)分,同時(shí)測(cè)定血清白細(xì)胞介素-1β、白細(xì)胞介素-6水平及血液黏度,并在光鏡下觀察其組織形態(tài)及炎性細(xì)胞的浸潤(rùn)情況。結(jié)果:①軟組織損傷評(píng)分。治療組軟組織損傷評(píng)分低于模型組(t=8.760,P=0.000)。②白細(xì)胞介素-1β。各組血清白細(xì)胞介素-1β含量比較,差異有統(tǒng)計(jì)學(xué)意義(F=84.380,P=0.000)。組間兩兩比較,治療組、模型組含量高于正常組(P=0.001,P=0.000),治療組低于模型組(P=0.000)。③白細(xì)胞介素-6。各組血清白細(xì)胞介素-6含量比較,差異有統(tǒng)計(jì)學(xué)意義(F=118.781,P=0.000)。組間兩兩比較:正常組、治療組低于模型組(P=0.000,P=0.000); 治療組與正常組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.838)。④血液黏度。3組間全血高切黏度、中切黏度、低切黏度及血漿黏度比較,差異有統(tǒng)計(jì)學(xué)意義(F=106.650,P=0.000; F=169.403,P=0.000; F=145.910,P=0.000; F=23.972,P=0.000)。組間兩兩比較,治療組、模型組全血高切黏度、中切黏度、低切黏度及血漿黏度均高于正常組(P值均為0.000),而治療組各指標(biāo)均低于模型組(P值均為0.000)。⑤組織形態(tài)學(xué)觀察。正常組肌纖維形態(tài)較一致,肌細(xì)胞呈束狀平行緊密排列,細(xì)胞呈長(zhǎng)梭形,胞漿豐富; 模型組有的可見(jiàn)部分肌纖維腫脹,有的可見(jiàn)白細(xì)胞呈彌漫性浸潤(rùn); 治療組肌纖維間質(zhì)白細(xì)胞浸潤(rùn)不明顯,肌纖維形態(tài)大致正常。治療組創(chuàng)傷病灶中炎性細(xì)胞浸潤(rùn)密度低于模型組(t=14.655,P=0.000)。結(jié)論:消腫止痛膠囊能夠明顯減輕急性軟組織損傷部位的腫脹、瘀血,減少炎性細(xì)胞浸潤(rùn),降低全血黏度、血漿黏度及血清白細(xì)胞介素-1β和白細(xì)胞介素-6水平。其治療急性軟組織損傷的機(jī)制可能是降低血液黏度,并抑制炎性細(xì)胞因子白細(xì)胞介素-1β及白細(xì)胞介素-6的釋放。

Abstract:

Objective:To explore the curative effects and possible function mechanism of XIAOZHONG ZHITONG capsule on acute soft tissue injury.Methods:Thirty healthy New Zealand rabbits were randomly divided into normal group,model group and treatment group,10 cases in each group.Rabbits models with acute traumatic hematomas were built for all the groups except for normal group.Rabbits in the normal group and model group were lavaged with physiological saline,while the others in the treatment group were lavaged with XIAOZHONG ZHITONG suspension(20 mg·mL^-1),the dosage of each solution mentioned above was 4 mL·kg^-1.Five days after the modeling,the injury changes of the wounded limbs were observed by naked eyes and the results were scored according to the standards formulated by Feng Fang-jun.In the meantime,the levels of interleukin-1beta(IL-1β)and interleukin-6(IL-6)of blood serum and the blood viscosity were evaluated,and their histomorphometric situations and infiltratins of inflammatory cells were observed under light microscope.Results:①Scores of soft tissue injury:the scores of treatment group were lower than those of the model group(t=8.760,P=0.000).②IL-1β:there was statistical difference in IL-1β content of blood serum among the 3 groups(F=84.380,P=0.000).IL-1β contents of treatment group and model group were higher than those of normal group respectively(P=0.001,P=0.000),while the contents of treatment group were lower than those of model group(P=0.000).③IL-6:there was statistical difference in IL-6 content of blood serum among the 3 groups(F=118.781,P=0.000).IL-6 contents of normal group and treatment group were lower than those of model group respectively(P=0.000,P=0.000),while there was no statistical difference between treatment group and normal group(P=0.838).④Blood viscosity:there were statistical difference in the high shear viscosity of whole blood,medium shear viscosity of whole blood,low shear viscosity of whole blood and the plasma viscosity among the 3 groups(F=106.650,P=0.000; F=169.403,P=0.000; F=145.910,P=0.000; F=23.972,P=0.000).The high shear viscosity of whole blood,medium shear viscosity of whole blood,low shear viscosity of whole blood and the plasma viscosity of treatment group and model group were higher than those of normal group respectively(P=0.000),while all index above of treatment group were lower than model group(P=0.000).⑤Histomorphometric observation:such situations as unanimous shape of muscle fibers,tightly aligned parallel myocyte in sarciniform,fusiform cells and rich cells plasma were found in normal group; part of the muscle fiber swelling and diffuse infiltration of white blood cells were showed partly in model group; unconspicuous infiltration of muscle interstitial leukocyte and roughly normal shape of muscle fiber were found in treatment group.Infiltration density of inflammatory cells in the focus of traumatic lesions in the treatment group were lower than those of the model group(t=14.655,P=0.000).Conclusion:XIAOZHONG ZHITONG capsule can obviously relieve the situations of swelling and blood stasis for the parts of acute soft tissue injury,decrease the occurrence of infiltration for the inflammatory cells and lower down the blood viscosity,plasma viscosity and the levels of IL-1β and IL-6 in blood serum.The mechanism of XIAOZHONG ZHITONG capsule on acute soft tissue injury maybe rely on the decrease of blood viscosity and the inhibition of the release of such inflammatory cytokines as IL-1β and IL-6.

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更新日期/Last Update: 1900-01-01