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[1]廖懷章,游葉平,何樂善,等.膝骨痹康膠囊對膝骨關節(jié)炎大鼠腫瘤壞死因子-α、轉化生長因子-β1的影響[J].中醫(yī)正骨,2011,23(10):3-5.
　LIAO Huai-zhang*,YOU Ye-ping,HE Le-shan,et al.Effect of QIGUBIKANG capsules on tumor necrosis factor-alpha and transforming growth factor beta1 in the rats with knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(10):3-5.
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膝骨痹康膠囊對膝骨關節(jié)炎大鼠腫瘤壞死因子-α、轉化 生長因子-β1的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年10期
頁碼:
3-5
欄目:
基礎研究
出版日期:
2011-10-31

文章信息/Info

Title:
Effect of QIGUBIKANG capsules on tumor necrosis factor-alpha and transforming growth factor beta1 in the rats with knee osteoarthritis
作者:
廖懷章1; 游葉平1; 何樂善1; 孫煒1; 劉緒銀1; 李智雄2; 肖磊2; 田麗君2
1.湖南省邵陽正骨醫(yī)院,湖南 新邵 422900; 2.湖南中醫(yī)藥大學,湖南 長沙 410007
Author(s):
LIAO Huai-zhang*; YOU Ye-ping; HE Le-shan; et al.*
Shaoyang Orthopedic-Traumatological Hospital,Xinshao 422900,Hunan,China
關鍵詞:
骨關節(jié)炎; 膝 膝骨痹康膠囊 腫瘤壞死因子α 轉化生長因子β1
Keywords:
Osteoarthritis; knee;  QIGUBIKANG capsules;  Tumor necrosis factor-alpha;  Transforming growth factor beta1
摘要:
目的:通過研究膝骨痹康膠囊對膝骨關節(jié)炎大鼠腫瘤壞死因子-α、轉化生長因子-β1的影響,探討膝骨痹康膠囊治療膝骨關節(jié)炎的作用機制。方法:取60只健康清潔級SD大鼠,隨機選取10只納入空白對照組,其余50只采用單側跟腱切除法制造大鼠膝骨關節(jié)炎模型,造模成功后將其隨機分為模型對照組、抗骨質(zhì)增生膠囊組、膝骨痹康膠囊低劑量組、膝骨痹康膠囊中劑量組、膝骨痹康膠囊高劑量組,每組10只。藥物干預30 d后采用免疫組織化學法觀察大鼠膝關節(jié)軟骨中腫瘤壞死因子-α、轉化生長因子β1的表達。結果:①腫瘤壞死因子-α。各組陽性細胞平均光密度比較,差異有統(tǒng)計學意義(F=9.740,P=0.000)。組間兩兩比較:空白對照組平均光密度小于模型對照組、膝骨痹康膠囊低劑量組和中劑量組(P=0.000; P=0.001; P=0.012),模型對照組平均光密度大于抗骨質(zhì)增生膠囊組、膝骨痹康膠囊低劑量組、中劑量組和高劑量組(P=0.000; P=0.015; P=0.001; P=0.000),膝骨痹康膠囊低劑量組平均光密度大于高劑量組(P=0.024),其余各組比較,差異均無統(tǒng)計學意義。②轉化生長因子-β1。各組陽性細胞平均光密度比較,差異有統(tǒng)計學意義(F=11.526,P=0.000)。組間兩兩比較:空白對照組平均光密度大于其余5組(P=0.000; P=0.000; P=0.001; P=0.036; P=0.000),模型對照組平均光密度小于膝骨痹康膠囊低劑量組和中劑量組(P=0.011; P=0.000),抗骨質(zhì)增生膠囊組平均光密度小于膝骨痹康膠囊中劑量組(P=0.011),膝骨痹康膠囊中劑量組平均光密度大于高劑量組(P=0.007),其余各組比較,差異均無統(tǒng)計學意義。結論:膝骨痹康膠囊可能是通過下調(diào)膝骨關節(jié)炎患者關節(jié)軟骨中腫瘤壞死因子-α的表達,同時上調(diào)轉化生長因子β1的表達來發(fā)揮治療作用的。
Abstract:
Objective:To explore the mechanism of action of QIGUBIKANG capsules in the treatment of knee osteoarthritis(KOA)through the study of its effect on tumor necrosis factor-alpha(TNF-α)and transforming growth factor beta1(TGF-β1)in the rats with knee osteoarthritis.Methods:Ten cases were randomly selected from the sixty healthy SD rats of clean grade and were enrolled into the blank control group,while the others were made into KOA models with excision of unilateral achilles tendon.After the successful modeling,the 50 KOA models were randomly divided into model control group,anti-hyperostosis capsules group,low-dose-QIGUBIKANG-capsule group,medium-dose-QIGUBIKANG-capsule group and high-dose-QIGUBIKANG-capsule group,10 cases in each group.After 30 days of drug intervention,the expressions of TNF-α and TGF-β1 in rat knee cartilage were observed through immunohistochemical method.Results:①TNF-α:There was statistical difference in the average optical of positive cells among the 6 groups(F=9.740,P=0.000); the average optical of positive cells in the blank control group was smaller than that of model control group,low-dose-QIGUBIKANG-capsule group and medium-dose-QIGUBIKANG-capsule group respectively(P=0.000; P=0.001; P=0.012),and the average optical of positive cells in the model control group was higher than that of anti-hyperostosis capsules group, low-dose-QIGUBIKANG-capsule group,medium-dose-QIGUBIKANG-capsule group and high-dose-QIGUBIKANG-capsule group respectively(P=0.000; P=0.015; P=0.001; P=0.000); and the average optical of positive cells in the low-dose-QIGUBIKANG-capsule group was higher than that of high-dose-QIGUBIKANG-capsule group(P=0.024); while there was no statistical difference in the average optical of positive cells compared between any other groups.②TGF-β1:There was statistical difference in the average optical of positive cells among the 6 groups(F=11.526,P=0.000); the average optical of positive cells in the blank control group was higher than that of the other 5 groups respectively(P=0.000; P=0.000; P=0.001; P=0.036; P=0.000); the average optical of positive cells in model control group was smaller than that of low-dose-QIGUBIKANG-capsule group and medium-dose-QIGUBIKANG-capsule group respectively(P=0.011; P=0.000),and the average optical of positive cells in anti-hyperostosis capsules group was smaller than that of medium-dose-QIGUBIKANG-capsule group(P=0.011),and the average optical of positive cells in medium-dose-QIGUBIKANG-capsule group was higher than that of high-dose-QIGUBIKANG-capsule group(P=0.007); while there was no statistical difference in the average optical of positive cells compared between any other groups.Conclusion:QIGUBIKANG capsules can exert its therapeutic effects on KOA through the down-regulation of TNF-α expression in joint cartilages and the up-regulation of TGF-β1 expression.

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更新日期/Last Update: 1900-01-01