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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:

第23卷

期數(shù):

2011年09期

頁(yè)碼:

14-16

欄目:

基礎(chǔ)研究

出版日期:

2011-09-30

文章信息/Info

Title:

Experimental study on the anti-inflammation and analgesia effects of JINHUANG cream

作者:

徐華明; 江國(guó)榮; 張露蓉; 梁國(guó)強(qiáng)

江蘇省蘇州市中醫(yī)醫(yī)院,江蘇 蘇州 215003

Author(s):

XU Hua-ming^*; JIANG Guo-rong; ZHANG Lu-rong; et al.^*

Traditional Chinese Medical Hospital of Suzhou City,Suzhou 215003,Jiangsu,China

關(guān)鍵詞:

劑型改進(jìn) 金黃乳膏 抗炎藥(中藥)鎮(zhèn)痛 動(dòng)物實(shí)驗(yàn)

Keywords:

Dosage form improving;  JINHUANG cream;  Anti-inflammatory drugs(TCD);  Analgesia;  Animal experimentation

摘要:

目的:評(píng)價(jià)金黃乳膏的抗炎鎮(zhèn)痛作用,探討其作為金黃油膏取代產(chǎn)品的可行性。方法:①直接給藥的抗炎作用。取小鼠28只,隨機(jī)分為模型組、扶他林乳膏組、金黃油膏組、金黃乳膏組,每組7只。先用二甲苯致小鼠耳廓腫脹,再將相應(yīng)藥物敷于各組小鼠致炎側(cè)耳部,隨后處死小鼠計(jì)算耳腫脹度。②間接給藥的抗炎作用。取小鼠24只,隨機(jī)分成模型組、扶他林乳膏組、金黃乳膏組,每組8只。先將相應(yīng)藥物敷于各組小鼠腹部并固定,1.5 h后用二甲苯致小鼠耳廓腫脹,隨后處死小鼠,測(cè)量并計(jì)算耳腫脹度。③不同載藥量金黃乳膏的抗炎作用。取小鼠37只,隨機(jī)分成模型組(n=7)、扶他林乳膏組(n=7)、金黃乳膏組(n=7)、金黃乳膏2.5倍量組(n=8)、金黃乳膏5倍量組(n=8)。先用二甲苯致小鼠耳廓腫脹,再將相應(yīng)藥物敷于各組小鼠致炎側(cè)耳部,隨后處死小鼠,測(cè)量并計(jì)算耳腫脹度。④鎮(zhèn)痛作用。取小鼠37只,隨機(jī)分為模型組(n=10)、扶他林乳膏組(n=9)、金黃油膏組(n=9)、金黃乳膏組(n=9)。先將相應(yīng)藥物敷于各組小鼠腹部并固定,連續(xù)給藥3 d。第3天給藥后2.5 h取下藥膏,按0.01 mL·g^-1體質(zhì)量腹腔注射0.9%冰醋酸。記錄注射后15 min內(nèi)小鼠的扭體次數(shù)。結(jié)果:①直接給藥后,扶他林乳膏組、金黃油膏組和金黃乳膏組的耳腫脹度均小于模型組(P=0.000,P=0.002,P=0.000),其余各組間比較,差異均無統(tǒng)計(jì)學(xué)意義; ②間接給藥后,金黃乳膏組和扶他林乳膏組耳腫脹度均小于模型組(P=0.004,P=0.003),金黃乳膏組與扶他林乳膏組比較,差異無統(tǒng)計(jì)學(xué)意義(P=0.057); ③測(cè)定不同載藥量金黃乳膏的抗炎作用時(shí),扶他林乳膏組、金黃乳膏組、金黃乳膏2.5倍量組和金黃乳膏5倍量組的腫脹度均小于模型組(P=0.000,P=0.000,P=0.000,P=0.000),其余各組間比較,差異均無統(tǒng)計(jì)學(xué)意義; ④測(cè)定金黃乳膏的鎮(zhèn)痛作用時(shí),扶他林乳膏組、金黃油膏組和金黃乳膏組的扭體次數(shù)均少于模型組(P=0.002,P=0.000,P=0.000),其余各組間比較,差異均無統(tǒng)計(jì)學(xué)意義。結(jié)論:金黃乳膏保留了金黃油膏的基本療效,對(duì)局部和全身都有良好的抗炎鎮(zhèn)痛效果。從藥效學(xué)角度來看,金黃乳膏作為金黃油膏的取代產(chǎn)品是可行的。

Abstract:

Objective:To evaluate the anti-inflammation and analgesia effects of JINHUANG cream,and to explore the feasibility of JINHUANG cream as the replacement product of JINHUANG ointment.Methods:①Anti-inflammation effects under direct administration:twenty eight mice were randomly divided into model group,voltaren cream group,JINHUANG ointment group and JINHUANG cream group,7 cases in each group.Ear swelling of mice were induced by xylene at first,and the inflammation parts of the ears were administrated with the relevant drugs,then the mice were sacrificed for the measurement of the ear swelling degrees.②Anti-inflammation effects under indirect administration:twenty four mice were randomly divided into model group,voltaren cream group and JINHUANG cream group,8 cases in each group.The relevant drugs were applied to and fixed on the abdomens of mice firstly,and 1.5 hours later,ear swelling of mice were induced by xylene,then the mice were sacrificed for the measurement of the ear swelling degrees.③Anti-inflammation effects of different dosage of JINHUANG cream:thirty seven mice were randomly divided into model group(n=7),voltaren cream group(n=7),JINHUANG cream group(n=7),2.5 times dosage of JINHUANG cream group(n=8)and 5 times dosage of JINHUANG cream group(n=8).Ear swelling of mice were induced by xylene at first,and the inflammation parts of the ears were administrated with the relevant drugs,then the mice were sacrificed for the measurement of the ear swelling degrees.④Analgesia effects:thirty seven mice were randomly divided into model group(n=10),voltaren cream group(n=9),JINHUANG ointment group(n=9)and JINHUANG cream group(n=9).At first,the relevant drugs were applied to and fixed on the abdomens of mice for 3 days continuously,and the plaster were taken down from the mice 2.5 hours after the administration in the 3rd day,then the mice were intraperitoneally injected with 0.9%glacial acetic acid(0.01 mL·g^-1).The writhing times of the mice in the first 15 minutes after the injection were recorded.Results:①After direct administration,ear swelling degrees of mice in voltaren cream group,JINHUANG ointment group and JINHUANG cream group were all lower than those of mice in model group(P=0.000,P=0.002,P=0.000),and there was no statistical difference between any other couple of groups.②After indirect administration,ear swelling degrees of mice in JINHUANG cream group and voltaren cream group were lower than those of mice in model group respectively(P=0.004,P=0.003),and there was no statistical difference between JINHUANG cream group and voltaren cream group(P=0.057).③The measurement result of the anti-inflammation effects of different dosage of JINHUANG cream showed that the ear swelling degrees of mice in voltaren cream group,JINHUANG cream group,2.5 and 5 times dosage of JINHUANG cream group were all lower than those of mice in model group(P=0.000,P=0.000,P=0.000,P=0.000),and there was no statistical difference between any other couple of groups.④The measurement result of the analgesia effects of JINHUANG cream showed that the writhing times of mice in voltaren cream group,JINHUANG ointment group and JINHUANG cream group were all less than those of mice in model group(P=0.002,P=0.000,P=0.000),and there was no statistical difference between any other couple of groups.Conclusion:JINHUANG cream retains the basic curative effects of JINHUANG ointment,and it has the satisfactory anti-inflammation and analgesia effects in the part and all over the body.From the pharmacodynamic point of view,it is feasible of JINHUANG cream to replace the JINHUANG ointment.

備注/Memo

備注/Memo:

2011-03-10收稿 2011-04-20修回

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更新日期/Last Update: 2011-09-30