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[1]戴七一,阮萍,袁經(jīng)陽,等.揉髕手法對(duì)兔膝關(guān)節(jié)軟骨增殖細(xì)胞核抗原表達(dá)的影響[J].中醫(yī)正骨,2011,23(08):6-8.
　DAI Qi-yi*,RUAN Ping,YUAN Jing-yang,et al.The Influence Of The Rou-Bin Manipulation To The PCNA Expression With Knee Joint Cartilage In Rabbit[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(08):6-8.
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揉髕手法對(duì)兔膝關(guān)節(jié)軟骨增殖細(xì)胞核抗原表達(dá)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年08期
頁碼:
6-8
欄目:
基礎(chǔ)研究
出版日期:
2011-08-30

文章信息/Info

Title:
The Influence Of The Rou-Bin Manipulation To The PCNA Expression With Knee Joint Cartilage In Rabbit
作者:
戴七一1; 阮萍1; 袁經(jīng)陽1; 覃學(xué)流1; 黎強(qiáng)2; 滕居贊1; 容向賓1;  韓杰1; 林光琪2; 劉靖2; 覃杰1; 王雄1; 吳兆沛1
1.廣西中醫(yī)學(xué)院附屬瑞康醫(yī)院,廣西 南寧 530011;
2.廣西中醫(yī)學(xué)院,廣西 南寧 530001
Author(s):
DAI Qi-yi*; RUAN Ping; YUAN Jing-yang; et al.*
Ruikang Hospital Affiliated to Guangxi Tradi tional Chinese Medical,Nanning 530011,Guangxi,China
關(guān)鍵詞:
手法 增殖細(xì)胞核抗原 膝關(guān)節(jié) 按摩 模型; 動(dòng)物 兔
Keywords:
Proliferating cell nuclear aneigen;  Knee joint;  Massage;  Models; Animal
摘要:
目的:觀察揉髕手法對(duì)兔膝關(guān)節(jié)軟骨增殖細(xì)胞核抗原表達(dá)的影響,進(jìn)而探討揉髕手法治療膝關(guān)節(jié)骨性關(guān)節(jié)炎的可能作用機(jī)制。方法:采用新西蘭兔50只,隨機(jī)分為手法組、針劑組、模型對(duì)照組、假模組和正常組各10只。前3組通過手術(shù)結(jié)扎并切斷右側(cè)臀下靜脈、股靜脈和大隱靜脈造模,假模組顯露相應(yīng)血管但不結(jié)扎,正常組不做任何處理。造模1周后手法組采用揉髕手法分別施術(shù)于兔右下肢5周共17次; 針劑組右膝關(guān)節(jié)內(nèi)每周注射1次玻璃酸鈉注射液,連用5周。其余3組不做任何處理。造模后8周末處死所有動(dòng)物,取右膝關(guān)節(jié)內(nèi)側(cè)脛骨平臺(tái)軟骨組織,采用免疫組化法檢測(cè)軟骨細(xì)胞增殖率,對(duì)所得數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)處理。結(jié)果:模型組軟骨細(xì)胞減少,增殖率為(13.25±2.66)%; 手法組軟骨細(xì)胞增多,增殖率為(17.90±2.80)%; 針劑組軟骨細(xì)胞增多,增殖率為(17.13±4.09)%; 假模組軟骨細(xì)胞增多,增殖率為(12.63±2.92)%; 正常組軟骨細(xì)胞正常,增殖率為(7.75±1.39)%; 與模型組比較,手法組、針劑組和正常組P<0.01,或P<0.05,差異有統(tǒng)計(jì)學(xué)意義。結(jié)論:揉髕手法可促進(jìn)兔膝關(guān)節(jié)軟骨細(xì)胞增殖,降低軟骨組織的損傷。
Abstract:
Objective:To observe the Influence Of The Rou-Bin Manipulation To The PCNA Expression With Knee Joint Cartilage In Rabbit, explore the mechanism of action of Rou-Bin manipulation to the knee osteoarthritis.Methods:50 New Zealand rabbits devided randomly into five gourps: Manipulation group, Inject group, Model group, dummy-model group and the normal group,10 rabbits in each group; ligaturing and cutting off the inferior-gluteal vein, femoral vein and great saphenous vein on the right side of the rabbit for the first 3 groups, shows the same veins without cutting off for dummy-model group, no treatment for the normal group. 1 week after modeled, 17 times of Rou-Bin manipulation on each rabbit in manipulation group, the rabbit injection with sodium hyaluronate 5 times in inject group, no special treatment for other rabbits. The 8th weekend after modeled, to take all the rabbit right knee medial tibial plateau cartilage tissue processing, detected chondrocyte proliferation rate by IHCA, statistics processing.Results:The amount of model group chondrocyte decreases, with a proliferation rate(13.25±2.66)%; The amount of manipulation group chondrocyte increase, with a proliferation rate(17.90±2.80)%; The amount of inject group chondrocyte increase, with a proliferation rate(17.13±4.09)%; The amount of dummy-model group chondrocyte increase, with a proliferation rate( 12.63±2.92)%; The normal group remain appro the same level, with a proliferation rate(7.75±1.39)%; To compare with model group, P<0.01(or P<0.05)for all of the manipulation group, inject group and normal group, has the highly significance difference.Conclusion:The Rou-Bin manipulation promote the chondrocyte proliferation rate of rabbit knee joint increase, reduce cartilage tissue damage.

參考文獻(xiàn)/References:

[1] 戴七一.手法治療髕骨軟骨病療效觀察及機(jī)理探討[J].廣西中醫(yī)藥,2002,25(2):17-18.
[2] 戴七一,文宗振,林光琪,等.揉髕手法對(duì)兔膝關(guān)節(jié)軟骨細(xì)胞凋亡影響的超微結(jié)構(gòu)觀察[J].中國中醫(yī)骨傷科雜志, 2010,18(6):1-3.
[3] 胥少汀,葛寶豐,徐印坎.實(shí)用骨科學(xué)[M].北京:人民軍醫(yī)出版社,2008:1337-1343.
[4] 宋楠萌,桑建利,徐恒.增殖細(xì)胞核抗原的分子結(jié)構(gòu)及其生物學(xué)功能研究進(jìn)展[J].自然科學(xué)進(jìn)展,2006,16(10):1201-1209.
[5] Joseph A.Buckwalter,MD,MS Thomas A,等.骨科基礎(chǔ)科學(xué)[M].北京:人民衛(wèi)生出版社,2001,389-390.
[6] 代嶺輝,杜寧.關(guān)節(jié)軟骨損傷生物學(xué)修復(fù)的研究進(jìn)展[J].中國骨傷,2009,22(9):721.
[7] 婁思權(quán).骨關(guān)節(jié)炎的病理和發(fā)病因素[J].中華骨科雜志,1996,16(1):56-59.
[8] 戴七一,張坤賜,陳秋順,等.膝關(guān)節(jié)骨痹的分型與X線影像關(guān)系的臨床研究[J].中國中醫(yī)骨傷科雜志,2007,15(7):23-32.
[9] 孟和,顧志華.骨傷科生物力學(xué)[M].北京:人民衛(wèi)生出版社,2006:310.
[10] 郭開今,王業(yè)華, 周冰,等.靜脈回流對(duì)膝關(guān)節(jié)骨內(nèi)壓影響的動(dòng)態(tài)觀察[J].徐州醫(yī)學(xué)院學(xué)報(bào),1995,15(4):389.

備注/Memo

備注/Memo:
2010-12-21收稿 2011-04-15修回
基金項(xiàng)目:國家自然科學(xué)基金資助項(xiàng)目(編號(hào):30860359)廣西科技廳自然基金資助項(xiàng)目(編號(hào):0832178)
更新日期/Last Update: 2011-08-30