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[1]李英周,惠礽華.膝痹康含藥血清對(duì)兔軟骨細(xì)胞中MMP-3的影響[J].中醫(yī)正骨,2011,23(08):3-5.
　LI Ying-zhou*,HUI Reng-hua.*.The empirical study on the effect of blood serum contained with Xibikang interventing matrix metalloproteinase-3 of cartilage cell[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(08):3-5.
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膝痹康含藥血清對(duì)兔軟骨細(xì)胞中MMP-3的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年08期
頁(yè)碼:
3-5
欄目:
基礎(chǔ)研究
出版日期:
2011-08-30

文章信息/Info

Title:
The empirical study on the effect of blood serum contained with Xibikang interventing matrix metalloproteinase-3 of cartilage cell
作者:
李英周1; 惠礽華2
1.浙江省上虞市中醫(yī)院,浙江 上虞 312300;
2.江蘇省蘇州市中醫(yī)院,江蘇 蘇州 215003
Author(s):
LI Ying-zhou*; HUI Reng-hua.*
Traditional Chinese Medical Hospital of Shang yu City,Shangyu 312300,Zhejiang,China
關(guān)鍵詞:
膝痹康 軟骨細(xì)胞 基因表達(dá) MMP-3 兔
Keywords:
Chondrocytes;  Gene Expression;  Drugs; Chinese Herbal
摘要:
目的:探討膝痹康對(duì)IL-1誘導(dǎo)的兔軟骨細(xì)胞中MMP-3蛋白含量及MMP-3mRNA表達(dá)的影響。方法:把軟骨細(xì)胞隨機(jī)分為空白組(A組); 模型組(B組)及低、中、高劑量組(C、D、E組)并加以培養(yǎng)、干預(yù)。用酶聯(lián)接免疫吸附劑測(cè)定(ELISA)法檢測(cè)細(xì)胞MMP-3蛋白含量,再用兩步法RT-PCR法檢測(cè)MMP-3mRNA的表達(dá)水平。結(jié)果:空白對(duì)照組正常關(guān)節(jié)軟骨細(xì)胞MMP-3蛋白含量及MMP-3mRNA表達(dá)量低于模型對(duì)照組,低、中、高劑量組組MMP-3蛋白含量及MMP-3mRNA表達(dá)量相對(duì)于模型組明顯降低(P<0.01)。其中中劑量組MMP-3mRNA表達(dá)量最低(P<0.01)。結(jié)論:膝痹康含藥血清可以抑制MMP-3的蛋白含量和MMP-3mRNA的表達(dá)水平,這可能是膝痹康中藥延緩膝OA軟骨退變,防止骨性關(guān)節(jié)炎的重要作用機(jī)制之一。
Abstract:
Objective:We study the machanism of the protection of Xibikang Prescription to chondrocytes induced by IL-1.Methods:The number of chondrocytes gained from wet cartilage, viability, multiplication and morphologic changes were observed. Chondrocytes were divided into different groups, XBK was added to subculture cells and its influence on protein levels of matrix metalloproteinase-3 was examined by ELISA method, and the influence on matrix metalloproteinase-3 mRNA expression were examined by RT-PCR method. Results:The levels of matrix metalloproteinase-3 protein and matrix metalloproteinase-3 mRNA expression.of the model control group was higher than that of the blank control group(P<0.01). The levels of high, middle and low dosage of Xibikang Prescription groups was lower than that of the model control group(P<0.05). and middle dosage of Xibikang Prescription groups was the most marked diffience with the model control group(P<0.01).Conclusion:Xibikang can effectively inhibit the levels of matrix metalloproteinase-3 protein and matrix metalloproteinase-3 mRNA expression.It plays a role in the mechanism of chondrocyte protection. It is effective to knee osteoarthritis patients.

參考文獻(xiàn)/References:

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備注/Memo

備注/Memo:
2010-04-18收稿 2010-08-21修回
更新日期/Last Update: 2011-08-30