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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:

第23卷

期數(shù):

2011年06期

頁碼:

21-24

欄目:

基礎研究

出版日期:

2011-06-30

文章信息/Info

Title:

Observation on the MRI image and pathology image of the canine model with femoral head avascular necrosis induced by liquid nitrogen frozen

作者:

朱建龍; 趙紅昌

浙江省杭州市蕭山區(qū)第四人民醫(yī)院,浙江 杭州 311225

Author(s):

ZHU Jian-long^*; ZHAO Hong-chang.^*

The Fourth People's Hospital of XiaoshanDistrict in Hangzhou City,Hangzhou 311225,Zhejiang,China

關鍵詞:

股骨頭壞死 模型; 動物 狗 磁共振成像 病理過程 液氮冷凍法

Keywords:

Femur head necrosis;  Models; animal;  Dogs;  Magnetic resonance imaging;  Pathologic processes;  liquid nitrogen frozen

摘要:

目的:從MRI影像和病理表現(xiàn)2個方面研究液氮冷凍法誘導的犬股骨頭缺血性壞死模型。方法:選取12只健康成年雄性Beagle犬,通過手術暴露其右側股骨頭后用一鐵制漏斗套于股骨頭,將100～150 mL液態(tài)氮倒入漏斗內,并維持冷凍3 min,用37 ℃溫鹽水將股骨頭復溫。左側股骨作為對照。造模后即開始觀察實驗犬的一般情況,造模后1個月隨機選取6只Beagle犬進行雙側股骨頭MRI掃描及病理學檢測,造模后2個月對剩余6只Beagle犬進行雙側股骨頭MRI掃描和組織病理學檢測。結果:①MRI掃描。造模后1個月可見實驗側股骨頭內于T1W上出現(xiàn)均質低信號,T2W信號升高,STIR信號升高更為明顯,呈散在、點狀分布于股骨頭內; 關節(jié)腔內可見少量關節(jié)積液; 股骨頭形態(tài)規(guī)則,邊緣光滑,大小與對側正常的股骨頭一致。造模后2個月實驗側股骨頭內于T1W上呈低信號,T2W上呈略高信號,STIR上呈略高信號; 關節(jié)腔內積液; 股骨頭邊緣不規(guī)則,變扁,變小; 股骨頭碎裂。②組織病理學觀察。造模后1個月實驗側骨膜不完整,軟骨部分脫落,軟骨層變薄,軟骨細胞排列紊亂; 骨小梁稀疏變細,結構紊亂,有碎片出現(xiàn),骨細胞陷窩空疏,骨細胞核固縮,部分血管栓塞; 骨髓腔內造血組織明顯減少,細胞數(shù)目及網(wǎng)狀結構較正常稀疏,可見到大量脂肪細胞、多形核細胞、炎性細胞浸潤,脂肪細胞體積增大,有的融合成泡狀。造模后2個月實驗側關節(jié)面破壞嚴重,組織結構紊亂、破碎,關節(jié)軟骨表層脫落、碎裂,軟骨細胞排列紊亂,切線層變薄,同源軟骨細胞數(shù)減少,軟骨下潮線區(qū)變薄,鈣化帶與軟骨下骨小梁不連續(xù),呈“倒鐘乳”狀,軟骨內可見到破骨細胞性骨吸收,形成髓腔。軟骨下血管內可見血栓形成。骨基質中有大量的空骨陷窩,僅見少量骨細胞散在,細胞核固縮偏移。骨小梁稀疏、變細、不完整,出現(xiàn)微骨折,或骨小梁碎裂、紊亂,部分溶解呈片狀。破骨細胞散在其間,骨髓組織壞死明顯,有纖維組織增生修復,脂肪細胞肥大,也可見到其壞死、崩解后留下的空腔。骨髓內見出血區(qū),造血細胞減少,大量脂肪細胞聚集,部分融合成大泡。結論:液氮冷凍法誘導的犬股骨頭缺血性壞死模型符合股骨頭壞死病變的一般規(guī)律,具有良好的重復性,是股骨頭缺血性壞死的理想模型。

Abstract:

Objective:To study the canine model with femoral head avascular necrosis induced by liquid nitrogen frozen from the 2 aspects as MRI image and pathology image.Methods:Twelve healthy adult male beagle dogs were chosen for building models.Iiron funnel was set on the dog's right lateral femoral head exposed by operation,then 100 to 150 milliliter liquid nitrogen was infunded into the funnel and frozen state was maintained for 3 minutes,and next,the rewarming of femoral head was proceeded with 37℃ warm saline.The left lateral femoral head was served as the control.The general conditions of the experimental dogs were observed after modeling at once.One month after the modeling,6 beagle dogs were randomly chosen for MRI scanning and pathology testing in their bilateral femoral heads,and the same procedures were given to the other 6 dogs two months after the modeling.Results:①On the aspect of MRI scanning:one month after modeling,homogeneous low signals appeared on T1W images of the femoral head in experimental side,and signals enhanced on T2W images.Meanwhile,signals which scattered in a punctate pattern in the femoral head,enhanced more significantly on STIR images.A small amount of hydrops articuli was found within joint cavity.The shape of the femoral head was regular,while its edges were smooth and its size was consistent with the contralateral normal femoral head.Two months after modeling,low signals appeared on T1W images of the femoral head in experimental side,and slight hyperintensity signals appeared on T2W images and STIR images.There was hydrops articuli in joint cavity.The femoral head had irregular edges and appeared flat and smaller,or even broken into pieces.②On the aspect of pathological observation:one month after modeling,the following phenomenon appeared in the femoral head in experimental side as incomplete periosteum,partly loss of cartilage,thinner cartilage layer,disordered arrangement of cartilage cells; thinner and sparse trabecular bone with disordered structure and fragments; bone cells with loose and empty lacunes,karyopyknosis and partly embolismed blood vessel; significantly reduce of hematopoietic tissue in marrow cavity,less cells amounts and looser networks compared with the normal situations,appearance of infiltration with large amount of adipocyte,polymorphocyte and inflammatory cells,increased volume of adipocyte with some merged into bubbly shape.Two months after modeling,the following phenomenon appeared in the femoral head in experimental side as serious destruction of articular surface,disordered and shattered organization structure,abscission and fragmentation of articular cartilage surface,disordered arrangement of cartilage cells,thinner tangential layer,reduced amounts of isogenous chondrocytes,thinner tidal lines under the cartilage,discontinuation between calcified zone and subchondral trabecular bone which formed a shape as inverted stalactite,appearance of medullary cavity formed by osteoclastic bone resorption within cartilage; formation of thrombus in the subchondral vascular; large amount of empty bone lacunas and small amount of scattered bone cells in bone matrix,excursion and pyknosis of nucleus; sparse,thinner and incomplete bone trabecula with microfracture,or shattered and disordered bone trabecula with some merged into laminar shape; existence of scattered osteoclasts,prominent necrosis of myeloid tissue,appearance of fibroplasia repair,hypertrophy of fat cells with some of their left cavities after necrosis and disintegration; appearance of bleeding area in bone marrow,reduced amount of hematopoietic cells,aggregation of large amount of fat cells with some merged into bulla.Conclusion:The canine model with femoral head avascular necrosis induced by liquid nitrogen frozen,which is consistent with the general rule of femur head necrosis and is good in reproducibity,is a kind of ideal model for femoral head avascular necrosis.

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更新日期/Last Update: 2011-06-30