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[1]杜深星,徐守宇,儲利勝,等.細(xì)胞外三磷酸腺苷對大鼠橈骨骨折愈合的影響[J].中醫(yī)正骨,2011,23(05):27-31.
　DU Shen-xing*,XU Shou-yu,CHU Li-sheng,et al.Effect of extracellular ATP on the radial fracture repair in rats[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(05):27-31.
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細(xì)胞外三磷酸腺苷對大鼠橈骨骨折愈合的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年05期
頁碼:
27-31
欄目:
基礎(chǔ)研究
出版日期:
2011-05-30

文章信息/Info

Title:
Effect of extracellular ATP on the radial fracture repair in rats
作者:
杜深星1; 徐守宇1; 2; 儲利勝1; 何幫劍2
1.浙江中醫(yī)藥大學(xué),浙江 杭州 310053;
2.浙江中醫(yī)藥大學(xué)附屬第三醫(yī)院,浙江 杭州 310005
Author(s):
DU Shen-xing*;  XU Shou-yu;  CHU Li-sheng;  et al.*
Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053,Zhejiang,China
關(guān)鍵詞:
三磷酸腺苷 骨折 再生 大鼠
Keywords:
Adenosine Triphosphate;  Fracture;  Regeneration;  Rat
摘要:
目的:研究細(xì)胞外三磷酸腺苷(Adenosine triphosphate,ATP)對大鼠橈骨骨折愈合的影響。方法:100只SD大鼠隨機(jī)平均分成ATP組和對照組,各組大鼠均采用顧氏造模法造成右側(cè)橈骨骨折模型。ATP組于造模時斷端注入2 mmoL·L-1的ATP,術(shù)后第1天、1周、2周、4周斷端注入4 mmoL·L-1的ATP,劑量均為0.05 mL·100 g-1,對照組斷端注入等體積生理鹽水。術(shù)后第1周、2周、4周、6周取材,進(jìn)行放射學(xué)、組織學(xué)、生物力學(xué)檢查。結(jié)果:放射學(xué)檢查術(shù)后第4周ATP組大多數(shù)骨折線趨于消失,其中有60%的標(biāo)本顯示骨折線完全消失,骨痂密度與骨密質(zhì)基本相同,達(dá)到骨性愈合; 對照組骨折線模糊,骨折斷端有骨痂填充,且大多數(shù)已橋接。術(shù)后第6周ATP組骨折線基本消失,骨痂密度與骨密質(zhì)基本相同,達(dá)到骨性愈合; 對照組骨折線趨于消失,骨折斷端被骨痂填充并橋接。組織學(xué)檢查術(shù)后第4周ATP組骨折斷端骨性骨痂融合形成板層骨,不規(guī)則骨髓腔亦融合形成新的再通的骨髓腔,達(dá)到骨性愈合; 對照組斷端可見大量骨性骨痂,形成不規(guī)則的骨髓腔。生物力學(xué)測定術(shù)后第4周和第6周ATP組骨痂的最大載荷、最大位移和彈性模量與對照組比較,差異有統(tǒng)計(jì)學(xué)意義(P<0.01)。結(jié)論:細(xì)胞外ATP具有促進(jìn)骨折愈合的作用。
Abstract:
Objective:To study the effect of extracellular ATP on the radial fracture repair in rats.Methods:100 SD rats were randomly divided into two groups: the ATP group(n=50)and the control group(n=50). All rats were established into right radius fracture rats models and the ATP group were injected 2 mmoL·L-1 ATP(0.05 mL·100 g-1)through fracture site in the same time. Then the ATP group were injected 4 mmol·L-1 ATP(0.05 mL·100 g-1)on these days: the day after the operation, 1 week after the operation, 2 weeks after the operation and 4 weeks after the operation. The control group were injected equal volume of NS through fracture site on the time listed above. All the rats were taken postoperative radiographic examination, histological examination and biomechanics measurements on these days: 1 week after the operation, 2 weeks after the operation, 4 weeks after the operation and 6 weeks after the operation.Results:The result of postoperative radiographic examination for the ATP group 4 weeks after the operation was that most fracture line tended to disappear and 60% of rat models in the ATP group showed a complete disappearance of their fracture lines with the density of poroma closing to the bone cortex which means the bones were basically healed. The control group's fracture lines tended to be blurred. The fracture site was filled with callus and most of them had already been bridged. After 6 weeks, the fracture lines of ATP group were disappeared basically with the density of poroma closing to the bone cortex. Fracture lines of the control group tend to disappear. The fracture site was filled with callus and had been bridged. After 4 weeks, histological examination showed that the osseouscallus formed into lamellar bone, and the marrow cavity reascularized. The fracture site of the control group showed that a lot of osseouscallus formed into irregular bone medullary cavity. Biomechanics measurements after 4 weeks and 6 weeks show that the poroma of the ATP group has a maximum of load, the biggest displacement and the greatest elastic modulus(P<0.01)compared to the control group.Conclusion:Extracellular ATP can promote fracture repairment.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省科技廳“新苗人才計(jì)劃”資助項(xiàng)目(2010R410010)
通訊作者:儲利勝 E-mail:[email protected]
更新日期/Last Update: 2011-05-30