84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]張雷,楊國(guó)敬,王珺,等.非創(chuàng)傷性股骨頭缺血性壞死差異表達(dá)蛋白 的鑒定和分析[J].中醫(yī)正骨,2011,23(05):13-18.
　ZHANG Lei*,YANG Guo-jing,WANG Jun,et al.Proteomic analysis of non-traumatic osteonecrosis of the femoral head[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(05):13-18.
點(diǎn)擊復(fù)制

非創(chuàng)傷性股骨頭缺血性壞死差異表達(dá)蛋白 的鑒定和分析()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年05期
頁(yè)碼:
13-18
欄目:
基礎(chǔ)研究
出版日期:
2011-05-30

文章信息/Info

Title:
Proteomic analysis of non-traumatic osteonecrosis of the femoral head
作者:
張雷1; 楊國(guó)敬1; 王珺2; 鄭進(jìn)佑1; 吳立東3; 嚴(yán)世貴3; 林標(biāo)楊2
1.溫州醫(yī)學(xué)院附屬第三醫(yī)院,浙江 瑞安 325200;
2.浙江大學(xué)浙江加州國(guó)際納米技術(shù)研究院, 浙江 杭州 310029;
3.浙江大學(xué)醫(yī)學(xué)院附屬第二醫(yī)院,浙江 杭州 310009
Author(s):
ZHANG Lei*;  YANG Guo-jing;  WANG Jun;  et al.*
The Third Affiliated Hospital of Wenzhou Medical College, Rui'an 325200, Zhejiang, China
關(guān)鍵詞:
股骨頭壞死 蛋白質(zhì)組學(xué) 多維色譜 質(zhì)譜 差異表達(dá)蛋白質(zhì)
Keywords:
Femur Head Necrosis;  Proteomics;  Multi-dimensional liquid chromatography;  Mass spectrometry;  Differential expressed protein
摘要:
目的:應(yīng)用組織蛋白質(zhì)組學(xué)方法尋找與成人非創(chuàng)傷性股骨頭缺血性壞死相關(guān)的蛋白質(zhì)。方法:分別于非創(chuàng)傷性股骨頭缺血性壞死患者和新鮮股骨頸骨折患者的股骨頭中采集壞死骨組織樣本與正常骨組織樣本8份,采用四種不同的溶劑提取骨組織中的總蛋白,應(yīng)用多維液相色譜與串聯(lián)質(zhì)譜聯(lián)用技術(shù)對(duì)組織蛋白進(jìn)行分離鑒定和生物信息學(xué)分析。結(jié)果:壞死組織樣本和正常組織樣本中鑒定二肽段以上的高可信度蛋白質(zhì)數(shù)量分別為1233個(gè)和999個(gè),假陽(yáng)性率為0.9%。共鑒定154種3倍以上的差異蛋白質(zhì)。基因本體論蛋白功能分析和文獻(xiàn)檢索顯示34種蛋白質(zhì)與股骨頭缺血性壞死密切相關(guān)。Western免疫印跡證實(shí)了ONFH患者骨組織中GPCR26和ChST2表達(dá)量下調(diào),與質(zhì)譜結(jié)果一致。結(jié)論:非創(chuàng)傷性股骨頭缺血性壞死存在復(fù)雜的病理生理學(xué)過程。研究發(fā)現(xiàn)的34個(gè)差異表達(dá)蛋白可能成為股骨頭缺血性壞死早期臨床診斷的候選生物學(xué)標(biāo)志物。
Abstract:
Objective:To explore proteins associated with non-traumatic osteonecrosis of the femoral head in adults by using high throughput proteomic approach.Methods:Eight necrotic bone tissue samples were harvested from the femoral head of patients with osteonercrosis of the femoral head, and 8 normal bone tissue samples were collected as the control group in the same patients. After bone tissues were demineralized by HCl solution, total bone proteins were extracted sequentially by using four different lysis buffers. Thereafter, multi-dimensional liquid chromatography-tandem mass spectrometry(MDLC-MS/MS)were applied to separate and identify the extracted proteins, and bioinformatics analysis was carried out then by the software tools such as TurboSEQUEST and GoMiner. Results:The in-depth proteome analysis of necrotic and normal bone tissue revealed 1233 and 999 high-confidence proteins respectively based on two peptides minimum, with the a false-positive rate of 0.9%. 154 differential expressed proteins, the level of which were upregulated or downregulated at least 3 fold were identified by spectral counting, as compared with the normal samples. Enrichment analysis in Gene Ontology categories of the differentially expressed gene found that 34 differential expressed proteins were highly associated with osteonecrosis of the femoral head. Downregulated expression of GPCR26 and ChST2 was confirmed by Western Blot on the samples of ONFH.Conclusion:The pathogenesis of the non-traumatic osteonecrosis of the femoral head has been proved complicated and remains unknown. These identified differential expressed proteins might be promising biomarker candidates for early diagnosis of the devastating disease. The results of this study will affirmatively provide a new approach to understand the etiopathogenesis of the osteonecrosis of the femoral head.

參考文獻(xiàn)/References:

[1] Liu YF,Chen WM,Lin YF,et al.Type II collagen gene variants and inherited osteonecrosis of the femoral head [J].N Engl J Med,2005,352:2294-2301.
[2] Chang JD,Hur M,Lee SS,et al.Genetic background of nontraumatic osteonecrosis of the femoral head in the Korean population[J].Clin Orthop Relat Res,2008,466:1041-1046.
[3] Lee HJ,Choi SJ,Hong JM,et al.Association of a polymorphism in the intron 7 of the SREBF1 gene with osteonecrosis of the femoral head in Koreans[J].Ann Hum Genet,2009,73:34-41.
[4] Tan X,Cai D,Wu Y,et al.Comparative analysis of serum proteomes: discovery of proteins associated with osteonecrosis of the femoral head[J].Transl Res,2006,148:114-119.
[5] Wu RW,Wang FS,Ko JY,et al.Comparative serum proteome expression of osteonecrosis of the femoral head in adults[J].Bone,2008,43:561-566.
[6] Jiang X,Ye M,Liu G,et al.Method development of efficient protein extraction in bone tissue for proteome analysis[J].J Proteome Res,2007,6:2287-2294.
[7] ARCO(Association Research Circulation Osseous):Committee on terminology and classification[M].ARCO News,1992:41-46.
[8] Motomura G,Yamamoto T,Miyanishi K,et al.Bone marrow fat-cell enlargement in early steroid-induced osteonecrosis-a histomorphometric study of autopsy cases[J].Pathol Res Pract,2005,200:807-811.
[9] Rajab A,Kunze J,Mundlos S.Spondyloepiphyseal dysplasia Omani type:a new recessive type of SED with progressive spinal involvement[J].Am J Med Genet A,2004,126A:413-419.
[10] Thiele H,Sakano M,Kitagawa H,et al.Loss of chondroitin 6-O-sulfotransferase-1 function results in severe human chondrodysplasia with progressive spinal involvement[J].Proc Natl Acad Sci U S A,2004,101(27):10155-10160.
[11] Hermanns P,Unger S,Rossi A,et al.Congenital joint dislocations caused by carbohydrate sulfotransferase 3 deficiency in recessive Larsen syndrome and humero-spinal dysostosis[J].Am J Hum Genet,2008,82:1368-1374.
[12] Takahashi S,Reddy SV,Chirgwin JM,et al.Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption[J].J Biol Chem,1994,269:28696-28701.
[13] Baldwin C,Nolan VG,Wyszynski DF,et al.Association of klotho,bone morphogenic protein 6,and annexin A2 polymorphisms with sickle cell osteonecrosis[J].Blood,2005,106:372-375.
[14] Larsson T,Sommarin Y,Paulsson M,et al.Cartilage matrix proteins.A basic 36-kDa protein with a restricted distribution to cartilage and bone[J].J Biol Chem,1991,266:20428-20433.
[15] Mizuno M,Fujisawa R,Kuboki Y.Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and shows affinity to collagen[J].Calcif Tissue Int,1996,59:163-167.
[16] Raouf A,Ganss B,McMahon C,et al.Lumican is a major proteoglycan component of the bone matrix[J].Matrix Biol,2002,21:361-367.
[17] Svensson L,Narlid I,Oldberg A.Fibromodulin and lumican bind to the same region on collagen type I fibrils[J].FEBS Lett,2000,470:178-182.

備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省醫(yī)藥衛(wèi)生科學(xué)研究基金(2007B207)溫州市科技計(jì)劃項(xiàng)目(Y20080006)
更新日期/Last Update: 2011-05-30