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[1]陳海嘯,梁軍波,伍海昭.骨碎補(bǔ)總黃酮對去卵巢大鼠腰椎松質(zhì)骨 骨形態(tài)計(jì)量學(xué)參數(shù)的影響[J].中醫(yī)正骨,2011,23(04):18-21.
　CHEN Hai-xiao*,LIANG Jun-bo,WU Hai-zhao.*.Effect of drynaria fortunei flavonoids on bone histomorphometry parameters of lumbar cancellous bone in ovariectomized rat[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(04):18-21.
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骨碎補(bǔ)總黃酮對去卵巢大鼠腰椎松質(zhì)骨 骨形態(tài)計(jì)量學(xué)參數(shù)的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年04期
頁碼:
18-21
欄目:
基礎(chǔ)研究
出版日期:
2011-04-30

文章信息/Info

Title:
Effect of drynaria fortunei flavonoids on bone histomorphometry parameters of lumbar cancellous bone in ovariectomized rat
作者:
陳海嘯; 梁軍波; 伍海昭
浙江省臺州醫(yī)院,浙江 臨海 317000
Author(s):
CHEN Hai-xiao*; LIANG Jun-bo; WU Hai-zhao.*
Taizhou Hospital of Zhejiang Province,Linhai 317000,Zhejiang China
關(guān)鍵詞:
骨質(zhì)疏松 骨碎補(bǔ) 骨形態(tài)計(jì)量學(xué) 動物實(shí)驗(yàn)
Keywords:
Osteoporosis;  Drynaria fortunet;  Bone histomorphometry;  Animal experimentation
摘要:
目的:研究骨碎補(bǔ)總黃酮對去卵巢大鼠腰椎松質(zhì)骨骨形態(tài)計(jì)量學(xué)參數(shù)的影響。方法:將40只SD大鼠隨機(jī)分為4組:假切除組(A組)、模型組(B組)、倍美力組(C組)、骨碎補(bǔ)組(D組),每組10只。麻醉后B、C、D組從背部入路切除雙側(cè)卵巢; A組僅切除腹部少許脂肪。造模后第2天開始,A、B組標(biāo)準(zhǔn)飼料常規(guī)飼養(yǎng); C組在標(biāo)準(zhǔn)飼料常規(guī)喂養(yǎng)的同時,每天灌服倍美力懸劑2 mL(按人鼠體表面積換算,相當(dāng)于倍美力0.017 mg); D組在標(biāo)準(zhǔn)飼料喂養(yǎng)的同時,每天灌服中藥濃縮劑2 mL(按人鼠體表面積換算,相當(dāng)于骨碎補(bǔ)總黃酮0.02 g)。6個月后取大鼠的L2椎體進(jìn)行骨形態(tài)計(jì)量學(xué)檢查。結(jié)果:4組大鼠骨體積分?jǐn)?shù)、骨小梁厚度、骨小梁間距、骨小梁節(jié)點(diǎn)數(shù)及游離末端數(shù)比較,差異均有統(tǒng)計(jì)學(xué)意義(F=4.031,P=0.043; F=3.980,P=0.045; F=4.133,P=0.041; F=4.091,P=0.043; F=4.382,P=0.041)。同一指標(biāo)組間比較:①B組骨體積分?jǐn)?shù)低于A、C、D組(LSD-t=3.322,P=0.011; LSD-t=2.861,P=0.019; LSD-t=3.023,P=0.015); ②B組骨小梁厚度低于A、C、D組(LSD-t=3.360,P=0.011; LSD-t=2.925,P=0.019; LSD-t=3.066,P=0.017); ③B組骨小梁間距大于A、C、D組(LSD-t=3.417,P=0.011; LSD-t=2.981,P=0.019; LSD-t=3.108,P=0.013); ④B組骨小梁節(jié)點(diǎn)數(shù)少于A、C、D組(LSD-t=4.191,P=0.080; LSD-t=2.956,P=0.039; LSD-t=2.632,P=0.045); ⑤B組骨小梁游離末端數(shù)大于A、C、D組(LSD-t=4.021,P=0.008; LSD-t=2.237,P=0.048; LSD-t=2.510,P=0.038); ⑥C組與D組骨體積分?jǐn)?shù)、骨小梁厚度、骨小梁間距、骨小梁節(jié)點(diǎn)數(shù)、骨小梁游離末端數(shù)比較,差異均無統(tǒng)計(jì)學(xué)意義(LSD-t=0.138,P=0.868; LSD-t=0.116,P=0.901; LSD-t=0.186,P=0.712; LSD-t=0.239,P=0.611; LSD-t=0.381,P=0.589)。結(jié)論:骨碎補(bǔ)總黃酮能通過增加骨量、改善骨三維結(jié)構(gòu),從而增強(qiáng)骨強(qiáng)度。
Abstract:
Objective:To study the effect of drynaria fortunei flavonoids on bone histomorphometry parameters of lumbar cancellous bone in ovariectomized rat.Methods:40 SD rats were randomly divided into 4 groups as sham-resection group(group A),model group(group B),premarin group(group C)and drynaria fortunei group(group D),10 cases in each group.Bilateral ovaries of rats in group B,group C and group D were removed from back approach after anesthesia,while the others in group A were administrated with resection of a little fat in belly only.From the 2nd day after molding,rats in group A and group B were fed on standard fodder normally,rats in group C were gavaged with 2mL premarin suspension agent(equivalent to 0.017mg premarin according to the conversion of human body surface area into rat body surface area )as well as fed on standard fodder every day,and rats in group D were gavaged with 2mL traditional Chinese medicine concentrate(equivalent to 0.02g drynaria fortunei flavonoids according to the conversion of human body surface area into rat body surface area )as well as fed on standard fodder every day.L2 centrums of rats were took for examination of bone morphometry after 6 months.Results:There was statistical difference in the following indexes as bone volume fraction,bone trabecular thickness,bone trabecular interval,number of bone trabecular nodes and number of bone trabecular free ends for the rats among the 4 groups(F=4.031,P=0.043; F=3.980,P=0.045; F=4.133,P=0.041; F=4.091,P=0.043; F=4.382,P=0.041).Comparison between groups for the same index:①Bone volume fraction of rats in group B were lower than those of rats in group A,group C and group D(LSD-t=3.322,P=0.011; LSD-t=2.861,P=0.019; LSD-t=3.023,P=0.015); ②Bone trabecula of rats in group B were thinner than those of rats in group A,group C and group D(LSD-t=3.360,P=0.011; LSD-t=2.925,P=0.019; LSD-t=3.066,P=0.017); ③Bone trabecular interval of rats in group B exceeded those of rats in group A,group C and group D(LSD-t=3.417,P=0.011; LSD-t=2.981,P=0.019; LSD-t=3.108,P=0.013); ④Bone trabecular nodes of rats in group B were less than those of rats in group A,group C and group D(LSD-t=4.191,P=0.080; LSD-t=2.956,P=0.039; LSD-t=2.632,P=0.045); ⑤Bone trabecular free ends of rats in group B were more than those of rats in group A,group C and group D(LSD-t=4.021,P=0.008; LSD-t=2.237,P=0.048; LSD-t=2.510,P=0.038); ⑥there was no statistical difference in bone volume fraction,bone trabecular thickness,bone trabecular interval,number of bone trabecular nodes and number of bone trabecular free ends of rats between group C and group D(LSD-t=0.138,P=0.868; LSD-t=0.116,P=0.901; LSD-t=0.186,P=0.712; LSD-t=0.239,P=0.611; LSD-t=0.381,P=0.589).Conclusion:Drynaria fortunei flavonoids can enhance bone strength through increasing bone mass and improving bone three-dimensional structure.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省衛(wèi)生廳基金項(xiàng)目(2005c098)
通訊作者:伍海昭 E-mail:[email protected]
更新日期/Last Update: 2011-04-30