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[1]熊輝,李前,談立明,等.桃紅四物湯有效成分的交互作用及其 對血清VEGF濃度的影響[J].中醫(yī)正骨,2011,23(02):3-6.
　XIONG Hui*,LI Qin,TAN Li-ming,et al.The interactions of Taohongsiwu Decoction's effective constituents and the influences on the VEGF protein expression of serum[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2011,23(02):3-6.
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桃紅四物湯有效成分的交互作用及其 對血清VEGF濃度的影響()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第23卷
期數(shù):
2011年02期
頁碼:
3-6
欄目:
基礎研究
出版日期:
2011-02-25

文章信息/Info

Title:
The interactions of Taohongsiwu Decoction's effective constituents and the influences on the VEGF protein expression of serum
作者:
熊輝1; 李前1; 談立明1; 楊臘平2
1.湖南中醫(yī)藥大學,湖南 長沙 410208;
2.湖南中醫(yī)藥大學第一附屬醫(yī)院,湖南 長沙 410007
Author(s):
XIONG Hui*; LI Qin; TAN Li-ming; et al.*
Hunan Vniversity of chinese Medicine,Changsha 410208,Hunan,China.
關(guān)鍵詞:
桃紅四物湯 有效成分 交互作用 骨折愈合 血清VEGF
Keywords:
TAOHONG SIWU DECOCTION;  effective constituents;  interaction;  healing of fracture;  callus;  histomorphology;  Vascular endothelial growth facfor(VEGF)of serum.
摘要:
目的:探討桃紅四物湯主次要有效成分的交互作用及其對血清VEGF濃度的影響。方法:從桃紅四物湯中提取4類有效成分:總生物堿(A)、總苷(B)、總多糖(C)和總揮發(fā)油(D),按照前期實驗所得結(jié)果(主要成分為A,次要為B、C、D),對清潔級小白鼠徒手定點折骨造模后進行實驗。按照L8(27)正交設計方案分組,觀察分析其對血清VEGF濃度的影響,探討主次要成分之間是否存在交互作用。結(jié)果:骨折造模后斷端局部出現(xiàn)VEGF蛋白高表達,提取物組表達量顯著高于模型組(P<0.05); 動態(tài)觀察結(jié)果,第2時相點(15 d)以內(nèi)表達量逐漸升高,在第15天出現(xiàn)表達峰值,此后表達量開始下降; 各組間比較,1～8組、10組3個時相點表達量均明顯高于9組; 在表達峰值(15 d)后VEGF表達量下降過程中,提取物及濃縮劑藥物使表達量下降程度減緩,使其在第3時相點(25 d)斷端骨痂組織內(nèi)仍維持高量VEGF蛋白表達。正交實驗表明,B藥取水平1與水平2差異有統(tǒng)計學意義(P<0.05),C藥和D藥取水平1與水平2差異無統(tǒng)計學意義(P>0.05)。結(jié)論:有效成分總生物堿與總苷有交互作用,與總多糖和總揮發(fā)油無交互作用; 總苷、總多糖與總揮發(fā)油之間也無交互作用。
Abstract:
Objective:To explore the interactions of Taohongsiwu Decoction's(THSWD's)effective constituents and the influences on the dynamic change of VEGF protein expression of serum.Methods:Based on the former experiment,we extracted 4 effective constituents from THSWD:A(total alkaloids TA)who is the principal,B(total glycosides TG),C(total polysaccharide TPS)and D(volatile oil TVO)who is the secondary, executed experiment on the mus musculus albus after bare-handed fixed-point bone snaped, layed 8 groups according to the orthogonal design proposal, analyzed the VEGF protein expression of serum,which in order to study on whether there is interactions on Taohongsiwu Decoction's(THSWD's)principal and secondary or not.Results:There was higher VEGF expression after fracture molding than in the model(P<0.05). The result of dynamic viewing indicated that VEGF expression quantity was more in 1～8 group and in 10 group than in 9 group(P<0.05),and the quantity of VEGF expression increased at the 15th day and reached the peak value. Then the quantity of VEGF expression decreased but more slowly in the extraction group,which made the higher concentrations of expression were maintained in the region of bone stump.The orthogonal experiment indicated that there was obvious difference between B's level 1 and level 2(P<0.05).But there was no difference between C's or D's level 1 and level 2(P>0.05).Conclusion:there was interactions between TA and TC,but there was no interactions among TA,TPS and TVO,and so did among TC,TPS and TVO.

參考文獻/References:

[1] 熊輝,左亞杰,丁志高,等.桃紅四物湯干預骨痂微血管新生及VEGF表達的實驗研究[J].中國醫(yī)師,2004,6(2):195-197.
[2] 柴本甫,過邦輔.理氣藥物對骨折愈合影響的初步研究[J].中華外科雜志,1962,1(10):299-304.
[3] Span PN,Grebenchtchikov N,Geurts-Moespot J,et al.EORTC Rceptor and Biomarker Study Group Report:a sandwich enzyme-linked immunosorbent assay for vascular endothelial growth factor in blood and tumor tissue extracts[J].Int J Biol Markers,2000,15(12):184-191.
[4] Clare R,Ozawa,Andrea B,et al.Microenviromental VEGF concentration,not total dose,determines a threshold between normal and aberrant angiogenesis[J].The Journal of Clinical Investigation,2004,133(4):516-527.

備注/Memo

備注/Memo:
基金項目:湖南省教育廳科學研究項目(編號:07C489)
更新日期/Last Update: 2011-02-25