84年鼠女哪年财运最旺,857comvvv色九欧美激情|85PO_87国产精品欲av国产av资源

[1]張力,張華,李平,等.中醫(yī)整脊棘突旁指壓法干預(yù)大鼠膝骨關(guān)節(jié)炎的效果及作用機(jī)制研究[J].中醫(yī)正骨,2025,37(03):14-22,38.
　ZHANG Li,ZHANG Hua,LI Ping,et al.Efficacy and mechanism of traditional Chinese medicine chiropractic paraspinous thumb-pressing therapy against knee osteoarthritis in rats:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2025,37(03):14-22,38.
點(diǎn)擊復(fù)制

中醫(yī)整脊棘突旁指壓法干預(yù)大鼠膝骨關(guān)節(jié)炎的效果及作用機(jī)制研究()
分享到：

《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第37卷
期數(shù):
2025年03期
頁(yè)碼:
14-22,38
欄目:
基礎(chǔ)研究
出版日期:
2025-03-20

文章信息/Info

Title:
Efficacy and mechanism of traditional Chinese medicine chiropractic paraspinous thumb-pressing therapy against knee osteoarthritis in rats:an experimental study
作者:
張力; 張華; 李平; 高嵩; 柯廣娟; 屈留新
東南大學(xué)附屬中大醫(yī)院,江蘇 南京 210009
Author(s):
ZHANG Li; ZHANG Hua; LI Ping; GAO Song; KE Guangjuan; QU Liuxin
Zhongda Hospital Affiliated to Southeast University,Nanjing 210009,Jiangsu,China
關(guān)鍵詞:
骨關(guān)節(jié)炎; 膝;  中醫(yī)整脊;  指壓法;  疼痛;  敏化;  瞬時(shí)受體電位香草素受體1;  神經(jīng)節(jié); 脊;  神經(jīng)元;  滑膜;  成纖維細(xì)胞;  串話;  大鼠
Keywords:
osteoarthritis; knee;  traditional Chinese medicine chiropractic;  thumb pressing manipulation;  pain;  sensitization;  transient receptor potential vanilloid 1;  ganglia; spinal;  neurons;  synovial membrane;  fibroblasts;  crosstalk;  rats
摘要:
目的:探討中醫(yī)整脊棘突旁指壓法干預(yù)大鼠膝骨關(guān)節(jié)炎(knee osteoarthritis,KOA)的效果及作用機(jī)制。方法:將40只SD大鼠隨機(jī)分為空白組、模型組、低強(qiáng)度組、中強(qiáng)度組和高強(qiáng)度組,每組8只。模型組、低強(qiáng)度組、中強(qiáng)度組和高強(qiáng)度組大鼠均采用碘乙酸鈉關(guān)節(jié)腔注射建立雙側(cè)KOA模型,空白組大鼠雙側(cè)膝關(guān)節(jié)腔注射等量生理鹽水。造模14 d后,采用小動(dòng)物計(jì)力指壓器于L3～L5棘突旁,對(duì)低、中、高強(qiáng)度組大鼠分別施以4 N、8 N、16 N的垂直向下壓力,模擬不同力度的棘突旁指壓法; 每次按壓2 s,每處連續(xù)按壓2 min; 每隔1 d施術(shù)1次,干預(yù)28 d; 空白組和模型組不進(jìn)行任何干預(yù)。分別于干預(yù)1 d、7 d、14 d、21 d、28 d,采用von Frey電子測(cè)痛儀測(cè)定大鼠機(jī)械刺激縮足閾值(paw withdrawal mechanical threshold,PWMT)。干預(yù)28 d后,采用ELISA試劑盒檢測(cè)大鼠血清中神經(jīng)生長(zhǎng)因子(nerve growth factor,NGF)、降鈣素基因相關(guān)肽(calcitonin gene related peptide,CGRP)含量; 采用鈣成像技術(shù)檢測(cè)大鼠L3背根神經(jīng)節(jié)神經(jīng)元Ca2+通量; 采用Western blot法檢測(cè)大鼠L3背根神經(jīng)節(jié)組織中瞬時(shí)受體電位香草素受體1(transient receptor potential vanilloid 1,TRPV1)、脂蛋白磷酸酶2B(protein phosphatases 2B,PP2B)、鈣調(diào)蛋白(calmodulin,CaM)的蛋白表達(dá)水平; 分別采用HE染色試劑盒、Masson染色試劑盒、天狼星紅染色試劑盒對(duì)大鼠膝關(guān)節(jié)滑膜組織進(jìn)行染色,觀察滑膜組織的炎性細(xì)胞浸潤(rùn)和纖維化情況; 采用Western blot法檢測(cè)大鼠膝關(guān)節(jié)滑膜組織中白細(xì)胞介素(interleukin,IL)-1β、腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)、轉(zhuǎn)化生長(zhǎng)因子(transforming growth factor,TGF)-β、α-平滑肌肌動(dòng)蛋白(α-smooth muscle actin,α-SMA)的蛋白表達(dá)水平。結(jié)果:①PWMT測(cè)定結(jié)果。空白組、模型組、低強(qiáng)度組大鼠PWMT隨時(shí)間變化均基本保持不變(F=0.885,P=0.487; F=0.339,P=0.849; F=0.555,P=0.697),中、高強(qiáng)度組大鼠PWMT隨時(shí)間變化均呈上升趨勢(shì)(F=8.222,P=0.000; F=9.844,P=0.000)。干預(yù)21 d、28 d,中、高強(qiáng)度組大鼠PWMT均高于模型組(干預(yù)21 d:P=0.002,P=0.001; 干預(yù) 28 d:P=0.003,P=0.000)和低強(qiáng)度組(干預(yù)21 d:P=0.022,P=0.011; 干預(yù)28 d:P=0.044,P=0.003),高強(qiáng)度組大鼠PWMT與中強(qiáng)度組比較,差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.774,P=0.273)。②血清疼痛介質(zhì)含量測(cè)定結(jié)果。中、高強(qiáng)度組大鼠血清NGF、CGRP含量均低于模型組(NGF含量:P=0.003,P=0.000; CGRP含量:P=0.000,P=0.000); 中強(qiáng)度組大鼠血清NGF含量與低強(qiáng)度組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=1.000),血清CGRP含量低于低強(qiáng)度組(P=0.000); 高強(qiáng)度組大鼠血清NGF、CGRP含量均低于低強(qiáng)度組(P=0.027,P=0.000); 高強(qiáng)度組大鼠血清NGF、CGRP含量與中強(qiáng)度組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=1.000,P=1.000)。③L3背根神經(jīng)節(jié)神經(jīng)元Ca2+通量檢測(cè)結(jié)果。低、中、高強(qiáng)度組大鼠L3背根神經(jīng)節(jié)神經(jīng)元Ca2+平均熒光強(qiáng)度均高于模型組(P=0.001,P=0.000,P=0.000),中、高強(qiáng)度組大鼠L3背根神經(jīng)節(jié)神經(jīng)元Ca2+平均熒光強(qiáng)度均高于低強(qiáng)度組(P=0.003,P=0.016),高強(qiáng)度組大鼠L3背根神經(jīng)節(jié)神經(jīng)元Ca2+平均熒光強(qiáng)度與中強(qiáng)度組比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.361)。④L3背根神經(jīng)節(jié)組織中TRPV1、PP2B、CaM的蛋白表達(dá)水平檢測(cè)結(jié)果。中、高強(qiáng)度組大鼠L3背根神經(jīng)節(jié)組織中TRPV1的蛋白相對(duì)表達(dá)量均高于模型組和低強(qiáng)度組(模型組:P=0.002,P=0.000; 低強(qiáng)度組:P=0.047,P=0.014),PP2B、CaM的蛋白相對(duì)表達(dá)量均低于模型組和低強(qiáng)度組(模型組:P=0.011,P=0.000; P=0.000,P=0.000; 低強(qiáng)度組:P=0.043,P=0.033; P=0.000,P=0.000); 高強(qiáng)度組大鼠L3背根神經(jīng)節(jié)組織中TRPV1、PP2B、CaM的蛋白相對(duì)表達(dá)量與中強(qiáng)度組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=1.000,P=1.000,P=1.000)。⑤膝關(guān)節(jié)滑膜組織病理學(xué)觀察結(jié)果。HE染色顯示,模型組大鼠滑膜組織中呈現(xiàn)明顯的炎性細(xì)胞浸潤(rùn),低、中、高強(qiáng)度組大鼠滑膜組織中炎性細(xì)胞浸潤(rùn)較模型組減輕,且中、高強(qiáng)度組減輕明顯; Masson染色顯示,模型組大鼠滑膜組織中膠原沉積較空白組明顯增加,低、中、高強(qiáng)度組大鼠滑膜組織中膠原沉積較模型組減少,且中、高強(qiáng)度組減少明顯; 天狼星紅染色顯示,模型組大鼠滑膜組織中Ⅱ型膠原明顯減少,低、中、高強(qiáng)度組大鼠滑膜組織中Ⅱ型膠原較模型組增多,且中、高強(qiáng)度組增多明顯。⑥膝關(guān)節(jié)滑膜組織中IL-1β、TNF-α、TGF-β、α-SMA的蛋白表達(dá)水平檢測(cè)結(jié)果。中、高強(qiáng)度組大鼠滑膜組織中IL-1β、TNF-α、TGF-β、α-SMA的蛋白相對(duì)表達(dá)量均低于模型組和低強(qiáng)度組(模型組:P=0.000,P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000,P=0.000; 低強(qiáng)度組:P=0.004,P=0.002,P=0.003,P=0.000; P=0.005,P=0.008,P=0.007,P=0.004); 高強(qiáng)度組大鼠滑膜組織中IL-1β、TNF-α、TGF-β、α-SMA的蛋白相對(duì)表達(dá)量與中強(qiáng)度組比較,組間差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.116,P=0.083,P=0.224,P=0.341)。結(jié)論:中醫(yī)整脊棘突旁指壓法干預(yù)大鼠KOA,能夠減輕機(jī)械性疼痛敏化和滑膜炎癥,且作用效果與力度有關(guān); 其作用機(jī)制可能與其能引起背根神經(jīng)節(jié)神經(jīng)元TRPV1脫敏及影響背根神經(jīng)節(jié)神經(jīng)元與滑膜成纖維細(xì)胞間串話有關(guān)。
Abstract:
Objective:To observe the effects of traditional Chinese medicine(TCM)chiropractic paraspinous thumb-pressing therapy against knee osteoarthritis(KOA)in rats,and to explore its underlying mechanism.Methods:Forty SD rats were selected and randomized into blank group,model group,low-intensity group,medium-intensity group and high-intensity group,with 8 ones in each group.All rats but the ones in blank group were modeled by using bilateral knee intra-articular injection of sodium iodoacetate for inducing KOA,while the ones in blank group by using injection of the same dosage of normal saline at the corresponding sites.Fourteen days after successful modeling,the rats in the low-,medium-,and high-intensity groups were subjected to 4 N,8 N,and 16 N vertical downward pressures,respectively,beside the L3-L5 spinous processes by using a force-measuring finger pressure device for small animals to simulate the paraspinous thumb-pressing manipulation with different intensities,once every other day,2 seconds per time and 2 minutes per site for consecutive 28 days; while the ones in blank group and model group were not given any intervention.The paw withdrawal mechanical threshold(PWMT)of rats were tested using a Von Frey electronic algometer after 1-,7-,14-,21- and 28-day intervention,respectively.After 28-day intervention,the levels of nerve growth factor(NGF)and calcitonin gene related peptide(CGRP)in serum were detected by using ELISA kit,and the Ca2+ flux in L3 dorsal root ganglion(DRG)neurons was detected by using Calcium imaging.Furthermore,the protein expression levels of transient receptor potential vanilloid 1(TRPV1),protein phosphatases 2B(PP2B)and calmodulin(CaM)in the L3 DRG tissues were detected using Western blot.After that,the rat knee synovial tissues were harvested and stained with HE staining kit,Masson staining kit,and Sirius red staining kit,respectively,to observe the inflammatory cell infiltration and fibrosis,and the protein expression levels of interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),transforming growth factor(TGF)-β,and α-smooth muscle actin(α-SMA)in the knee synovial tissues were detected using Western blot.Results:①The measuring results of PWMT.The PWMT remained essentially unchanged over time in blank group,model group,and low-intensity group(F=0.885,P=0.487; F=0.339,P=0.849; F=0.555,P=0.697),while that presented an upward trend over time in medium- and high-intensity groups(F=8.222,P=0.000; F=9.844,P=0.000).After 21- and 28-intervention,the PWMT was higher in medium- and high-intensity groups compared to model group and low-intensity group(21-intervention:P=0.002,P=0.001; P=0.022,P=0.011; 28-intervention:P=0.003,P=0.000; P=0.044,P=0.003),while,it was not significantly different from each other between high-intensity group and medium-intensity group(P=0.774,P=0.273).②The serum level of pain medi-ator.The serum levels of NGF and CGRP were lower in medium- and high-intensity groups compared to model group(NGF:P=0.003,P=0.000; CGRP:P=0.000,P=0.000).The serum level of CGRP was lower in medium-intensity group compared to low-intensity group(P=0.000),while,there was no statistical difference in the serum level of NGF between the 2 group(P=1.000).The serum levels of NGF and CGRP were lower in high-intensity group compared to low-intensity group(P=0.027,P=0.000),while,they were not significantly different from each other between high-intensity group and medium-intensity group(P=1.000,P=1.000).③The Ca2+ flux in L3 DRG neurons.The average fluorescence intensity of Ca2+ in L3 DRG neurons was higher in low-,medium-,and high-intensity groups compared to model group(P=0.001,P=0.000,P=0.000),and was higher in medium- and high-intensity groups compared to low-intensity group(P=0.003,P=0.016),while,it was not significantly different from each other between high-intensity group and medium-intensity group(P=0.361).④The protein expression levels of TRPV1,PP2B and CaM in the L3 DRG tissues.The relative protein expression level of TRPV1 in the L3 DRG tissues was higher,while that of PP2B and CaM was lower in medium- and high-intensity groups compared to model group and low-intensity group(model group:P=0.002,P=0.000; P=0.011,P=0.000; P=0.000,P=0.000; low-intensity group:P=0.047,P=0.014; P=0.043,P=0.033; P=0.000,P=0.000),while,there was no statistical difference in the relative protein expression levels of TRPV1,PP2B,and CaM between high-intensity group and medium-intensity group(P=1.000,P=1.000,P=1.000).⑤Pathological observation of knee synovial tissues.HE staining showed that,in model group,there was obvious inflammatory cell infiltration in the synovial tissues of rats,which was improved in low-,medium-,and high-intensity groups compared to model group,with a more pronounced improvement in medium- and high-intensity groups.Masson staining revealed that,compared to blank group,the collagen deposition in the synovial tissues significantly increased in rats of model group; compared to the model group,the collagen deposition decreased in low-,me-dium-,and high-intensity groups,with a more marked reduction in medium- and high-intensity groups.Sirius red staining showed that the typeⅡcollagen in the synovial tissues significantly decreased in rats of model group,while,compared to the model group,the typeⅡcollagen increased in low-,medium-,and high-intensity groups,with a more significant increase in medium- and high-intensity groups.⑥The protein expression levels of IL-1β,TNF-α,TGF-β and α-SMA in knee synovial tissues.The relative protein expression levels of IL-1β,TNF-α,TGF-β and α-SMA in knee synovial tissues were lower in medium- and high-intensity groups compared to model group and low-intensity group(model group:P=0.000,P=0.000,P=0.000,P=0.000; P=0.000,P=0.000,P=0.000,P=0.000; low-intensity group:P=0.004,P=0.002,P=0.003,P=0.000; P=0.005,P=0.008,P=0.007,P=0.004),while,they were not significantly different from each other between high-intensity group and medium-intensity group(P=0.116,P=0.083,P=0.224,P=0.341).Conclusion:The TCM chiropractic paraspinous thumb-pressing therapy can alleviate mechanical pain sensitization and synovial inflammation in KOA rats,and the effect exhibits intensity-dependence.It may work by inducing the desensitization of TRPV1 in DRG neurons and influencing the crosstalk between DRG neurons and synovial fibroblasts.

參考文獻(xiàn)/References:

[1] DUONG V,OO W M,DING C,et al.Evaluation and treatment of knee pain:a review[J].JAMA,2023,330(16):1568-1580.
[2] 屈留新.張朝純教授中醫(yī)整脊療法適應(yīng)證淺談[J].中國(guó)中醫(yī)骨傷科雜志,2021,29(4):82-84.
[3] 屈留新,王魯燁,邢麗陽(yáng),等.中醫(yī)整脊療法治療膝骨關(guān)節(jié)炎的臨床研究[J].南京中醫(yī)藥大學(xué)學(xué)報(bào),2019,35(2):152-155.
[4] QU L,XING L,NORMAN W,et al.Clinical effect of traditional Chinese spinal orthopedic manipulation in treatment of chondromalacia patellae[J].J Tradit Chin Med,2016,36(6):718-723.
[5] ROELOFS A J,DE BARI C.Osteoarthritis year in review 2023:biology[J].Osteoarthritis Cartilage,2024,32(2):148-158.
[6] ZHANG L,LI M,LI X,et al.Characteristics of sensory innervation in synovium of rats within different knee osteoarthritis models and the correlation between synovial fibrosis and hyperalgesia[J].J Adv Res,2021,35:141-151.
[7] 韋以宗,林遠(yuǎn)方,韋春德.中醫(yī)整脊技術(shù)古籍文獻(xiàn)考[J].中華中醫(yī)藥雜志,2021,36(4):1832-1835.
[8] 龍翔宇,王剛,王繼紅,等.健翔理筋滾法和拇指按壓與傳統(tǒng)手法電腦成像力學(xué)分析[J].內(nèi)蒙古中醫(yī)藥,2019,38(3):79-81.
[9] 張皞晟,王培民.一種小鼠背根神經(jīng)節(jié)原代神經(jīng)元細(xì)胞分離與培養(yǎng)的優(yōu)化方法[J].臨床神經(jīng)外科雜志,2023,20(2):189-194.
[10] LI W,LV Z,WANG P,et al.Near infrared responsive gold nanorods attenuate osteoarthritis progression by targeting TRPV1[J].Adv Sci(Weinh),2024,11(16):e2307683.
[11] ZHANG Y,MA S,KE X,et al.The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neurons[J].Cell Biosci,2021,11(1):167.
[12] GUO B C,KUO K L,CHEN C H,et al.Di-(2-ethylhexyl)phthalate limits the pleiotropic effects of statins in chronic kidney disease patients undergoing dialysis and endothelial cells[J].Environ Pollut,2020,267:115548.
[13] OBEIDAT A M,DONNER A,MILLER R E.An update on targets for treating osteoarthritis pain:NGF and TRPV1[J].Curr Treatm Opt Rheumatol,2020,6(3):129-145.
[14] 尚玉巧.TRPV1致敏和脫敏建立類風(fēng)濕關(guān)節(jié)炎熱痹與寒痹證候模型的實(shí)驗(yàn)研究[D].廣州:南方醫(yī)科大學(xué),2023.
[15] MCALINDON T E,BANNURU R R,SULLIVAN M C,et al.OARSI guidelines for the non-surgical management of knee osteoarthritis[J].Osteoarthritis Cartilage,2014,22(3):363-388.
[16] ARDEN N K,PERRY T A,BANNURU R R,et al.Non-surgical management of knee osteoarthritis:comparison of ESCEO and OARSI 2019 guidelines[J].Nat Rev Rheumatol,2021,17(1):59-66.
[17] ESANCY K,DHAKA A.Turning down the body heat:a novel mechanism for TRPV1 antagonist-induced hyperther-mia[J].Neuron,2024,112(11):1727-1729.
[18] XIE Y K,LUO H,ZHANG S X,et al.GPR177 in a-fiber sensory neurons drives diabetic neuropathic pain via WNT-mediated TRPV1 activation[J].Sci Transl Med,2022,14(639):eabh2557.
[19] NEUBERGER A,ODA M,NIKOLAEV Y A,et al.Human TRPV1 structure and inhibition by the analgesic SB-366791[J].Nat Commun,2023,14(1):2451.
[20] 姜楚洋,王兆南,姜洪亮,等.基于TRPV1探討黃五甘附膏治療膝骨關(guān)節(jié)炎外周炎性痛敏的作用機(jī)制[J].中國(guó)實(shí)驗(yàn)方劑學(xué)雜志,2024,30(14):97-106.
[21] BEN-SHAANAN T L,KNÖPPER K,DUAN L,et al.Dermal TRPV1 innervations engage a macrophage-and fibroblast-containing pathway to activate hair growth in mice[J].Dev Cell,2024,59(21):2818-2833.
[22] LUO X,CHEN O,WANG Z,et al.IL-23/IL-17A/TRPV1 axis produces mechanical pain via macrophage-sensory neuron crosstalk in female mice[J].Neuron,2021,109(17):2691-2706.

相似文獻(xiàn)/References:

[1]樊慶陽(yáng),任凱晶.定制3D打印切模輔助全膝關(guān)節(jié)置換術(shù)治療 膝骨關(guān)節(jié)炎合并股骨干骨折畸形愈合[J].中醫(yī)正骨,2015,27(11):37.
[2]劉曉雅,孫永強(qiáng),劉國(guó)杰.主動(dòng)快速康復(fù)鍛煉對(duì)全膝關(guān)節(jié)置換術(shù)后關(guān)節(jié)活動(dòng)度的影響[J].中醫(yī)正骨,2015,27(09):73.
[3]鄭春松,葉蕻芝,李西海,等.透骨消痛膠囊中補(bǔ)腎柔肝藥和活血祛風(fēng)藥治療 骨關(guān)節(jié)炎作用方式的計(jì)算機(jī)模擬比較[J].中醫(yī)正骨,2015,27(07):6.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Comparison of the mode of action of Bushen Rougan(補(bǔ)腎柔肝)drugs versus Huoxue Qufeng(活血祛風(fēng))drugs contained in Tougu Xiaotong Jiaonang(透骨消痛膠囊)for the treatment of osteoarthritis:A computer simulation study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):6.
[4]宋兵華,孫俊英,倪增良,等.全膝關(guān)節(jié)置換術(shù)前CT測(cè)量股骨后髁角的臨床意義[J].中醫(yī)正骨,2015,27(07):38.
[5]鄭春松,葉蕻芝,李西海,等.獨(dú)活寄生湯含藥血清對(duì)白細(xì)胞介素1β誘導(dǎo)的 退變關(guān)節(jié)軟骨細(xì)胞中基質(zhì)金屬蛋白酶 和環(huán)氧化酶2表達(dá)的影響[J].中醫(yī)正骨,2015,27(12):1.
　ZHENG Chunsong,YE Hongzhi,LI Xihai,et al.Impact of Duhuo Jisheng Tang(獨(dú)活寄生湯)medicated serum on expression of matrix metalloproteinase and cyclooxygenase 2 in degenerative articular chondrocytes induced by interleukin-1 beta[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):1.
[6]王金良,孫京濤,李玲,等.骨水泥聯(lián)合螺釘修復(fù)全膝關(guān)節(jié)置換術(shù)中 脛骨平臺(tái)內(nèi)側(cè)骨缺損[J].中醫(yī)正骨,2015,27(12):55.
[7]馮榮,王平,李炳奇,等.鈹針刺絡(luò)拔罐結(jié)合中藥口服治療膝骨關(guān)節(jié)炎合并 原發(fā)性血小板增多癥1例[J].中醫(yī)正骨,2015,27(12):73.
[8]蔡云仙.圍手術(shù)期耳穴按壓聯(lián)合平衡針療法 在全膝關(guān)節(jié)置換術(shù)后鎮(zhèn)痛中的應(yīng)用[J].中醫(yī)正骨,2015,27(06):41.
[9]張榮,王健.人工全膝關(guān)節(jié)置換術(shù)的圍手術(shù)期心理護(hù)理[J].中醫(yī)正骨,2015,27(05):77.
[10]喻長(zhǎng)純,楊明路,王戰(zhàn)朝.不同手術(shù)方式治療脛骨平臺(tái)骨折畸形愈合的體會(huì)[J].中醫(yī)正骨,2015,27(03):37.
[11]孟維娜,明立功,王新德,等.關(guān)節(jié)鏡下清理聯(lián)合腓骨近1/3段截骨治療膝骨關(guān)節(jié)炎[J].中醫(yī)正骨,2015,27(11):40.
[12]明立功,孟維娜,王新德,等.腓骨近端截骨治療內(nèi)側(cè)間室膝骨關(guān)節(jié)炎的近期療效觀察[J].中醫(yī)正骨,2015,27(10):25.
[13]張杰,王人彥,張玉柱.膝骨關(guān)節(jié)炎的治療進(jìn)展[J].中醫(yī)正骨,2015,27(10):68.
[14]梁朝,蔡靜怡,閆立,等.針刀療法改善膝骨關(guān)節(jié)炎早期疼痛癥狀的療效評(píng)價(jià)[J].中醫(yī)正骨,2015,27(09):9.
　LIANG Zhao,CAI Jingyi,YAN Li,et al.Evaluation of the curative effect of needle-knife therapy for relieving knee pain in patients with early knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):9.
[15]王建武,黨建軍,李強(qiáng),等.四聯(lián)療法治療膝骨關(guān)節(jié)炎[J].中醫(yī)正骨,2015,27(08):44.
[16]劉紅娟,郭會(huì)利,郭樹農(nóng).云克聯(lián)合中藥治療膝骨關(guān)節(jié)炎的護(hù)理[J].中醫(yī)正骨,2015,27(08):75.
[17]陳衛(wèi)衡.探索建立系統(tǒng)的膝骨關(guān)節(jié)炎中醫(yī)臨床科研范式 和理論體系[J].中醫(yī)正骨,2015,27(07):1.
[18]帥波,沈霖,楊艷萍,等.加味青娥丸治療膝骨關(guān)節(jié)炎的作用機(jī)制研究[J].中醫(yī)正骨,2015,27(07):15.
　SHUAI Bo,SHEN Lin,YANG Yanping,et al.Study on the mechanism of action of Jiawei Qing'e Wan(加味青娥丸)for the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):15.
[19]梅其杰,袁長(zhǎng)深,段戡,等.壯藥骨痹方燙熨聯(lián)合運(yùn)動(dòng)療法治療膝骨關(guān)節(jié)炎的臨床研究[J].中醫(yī)正骨,2015,27(07):27.
　MEI Qijie,YUAN Changshen,DUAN Kan,et al.Clinical study of the curative effect of hot compressing and rubbing with packet of Gubi Fang(骨痹方)combined with exercise therapy in the treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):27.
[20]王丹輝,張燕,劉麗娟,等.重組人Ⅱ型腫瘤壞死因子受體-抗體融合蛋白 關(guān)節(jié)腔注射聯(lián)合中藥薰洗治療膝骨關(guān)節(jié)炎的臨床研究[J].中醫(yī)正骨,2015,27(07):31.
　WANG Danhui,ZHANG Yan,LIU Lijuan,et al.Clinical study on intra-articular injection of TypeⅡrecombinant human tumor necrosis factor receptor-Fc fusion protein combined with Chinese herbal steaming and washing therapy for treatment of knee osteoarthritis[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2015,27(03):31.

備注/Memo

備注/Memo:
基金項(xiàng)目:東南大學(xué)附屬中大醫(yī)院中西醫(yī)協(xié)同醫(yī)學(xué)科研項(xiàng)目(2023zxyxt03)
通訊作者:屈留新 E-mail:[email protected]
更新日期/Last Update: 1900-01-01