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[1]張若谷,葛欽文,鄒凱奧,等.補(bǔ)腎活血方對(duì)腰椎間盤(pán)退變小鼠腰椎終板軟骨的影響及其作用機(jī)制[J].中醫(yī)正骨,2025,37(03):5-13.
　ZHANG Ruogu,GE Qinwen,ZOU Kaiao,et al.Effects and mechanism of Bushen Huoxue Fang(補(bǔ)腎活血方)on lumbar endplate cartilage in mice with intervertebral disc degeneration:an experimental study[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2025,37(03):5-13.
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補(bǔ)腎活血方對(duì)腰椎間盤(pán)退變小鼠腰椎終板軟骨的影響及其作用機(jī)制()
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《中醫(yī)正骨》[ISSN:1001-6015/CN:41-1162/R]

卷:
第37卷
期數(shù):
2025年03期
頁(yè)碼:
5-13
欄目:
基礎(chǔ)研究
出版日期:
2025-03-20

文章信息/Info

Title:
Effects and mechanism of Bushen Huoxue Fang(補(bǔ)腎活血方)on lumbar endplate cartilage in mice with intervertebral disc degeneration:an experimental study
作者:
張若谷; 葛欽文; 鄒凱奧; 厲駒; 金紅婷; 童培建; 王萍兒
浙江中醫(yī)藥大學(xué)第一臨床醫(yī)學(xué)院,浙江 杭州 310053
Author(s):
ZHANG Ruogu; GE Qinwen; ZOU Kaiao; LI Ju; JIN Hongting; TONG Peijian; WANG Pinger
The First Clinical Medical College of Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China
關(guān)鍵詞:
椎間盤(pán)退化;  腰椎;  補(bǔ)腎活血方;  終板軟骨;  小鼠;  動(dòng)物實(shí)驗(yàn)
Keywords:
intervertebral disc degeneration;  lumbar vertebrae;  Bushen Huoxue Fang;  endplate cartilage;  mice;  animal experimentation
摘要:
目的:探討補(bǔ)腎活血方對(duì)腰椎間盤(pán)退變小鼠腰椎終板軟骨的影響及其作用機(jī)制。方法:將18只10周齡雄性SPF級(jí)C57BL/6J小鼠隨機(jī)分為空白對(duì)照組、模型組、補(bǔ)腎活血方組,每組6只。模型組和補(bǔ)腎活血方組采用腰椎失穩(wěn)術(shù)構(gòu)建腰椎間盤(pán)退變模型。造模后第2天,補(bǔ)腎活血方組小鼠用補(bǔ)腎活血方中藥提取液灌胃,空白對(duì)照組和模型組小鼠用生理鹽水灌胃,各組小鼠均每天灌胃1次,連續(xù)灌胃8周。灌胃結(jié)束后,脫頸處死各組小鼠,切取小鼠L4和L5椎體,進(jìn)行Micro-CT分析,并計(jì)算腰椎間盤(pán)相對(duì)高度。常規(guī)進(jìn)行小鼠腰椎終板軟骨組織病理學(xué)觀察,計(jì)算小鼠的腰椎終板軟骨組織形態(tài)學(xué)評(píng)分,測(cè)定小鼠腰椎終板軟骨組織中Ⅱ型膠原蛋白α1鏈(collagen typeⅡalpha 1 chain,Col2α1)、基質(zhì)金屬蛋白酶-13(matrix metalloproteinase-13,MMP-13)和pSmad2的表達(dá)情況。結(jié)果:①小鼠腰椎Micro-CT分析結(jié)果。空白對(duì)照組和補(bǔ)腎活血方組小鼠腰椎終板軟骨骨化區(qū)域的骨體積分?jǐn)?shù)均低于模型組(P=0.000,P=0.022),骨小梁數(shù)量均少于模型組(P=0.002,P=0.001),腰椎間盤(pán)相對(duì)高度均高于模型組(P=0.000,P=0.000)。補(bǔ)腎活血方組小鼠腰椎終板軟骨骨化區(qū)域的骨體積分?jǐn)?shù)高于空白對(duì)照組(P=0.003),骨小梁數(shù)量、腰椎間盤(pán)相對(duì)高度與空白對(duì)照組的差異均無(wú)統(tǒng)計(jì)學(xué)意義(P=0.882,P=0.794)。②小鼠腰椎終板軟骨組織病理學(xué)觀察結(jié)果。空白對(duì)照組小鼠的腰椎終板軟骨細(xì)胞異質(zhì)性較小,軟骨厚度正常,骨化面積較小。與空白對(duì)照組相比,模型組小鼠的腰椎終板軟骨細(xì)胞異質(zhì)性顯著增大,軟骨明顯變薄,骨化面積增大。與模型組相比,補(bǔ)腎活血方組小鼠的腰椎終板軟骨細(xì)胞異質(zhì)性較小,軟骨厚度有所增加,骨化面積有所減小,但均未達(dá)到空白對(duì)照組的水平。空白對(duì)照組和補(bǔ)腎活血方組小鼠的腰椎終板軟骨組織形態(tài)學(xué)評(píng)分均低于模型組(P=0.000,P=0.000),空白對(duì)照組與補(bǔ)腎活血方組小鼠的腰椎終板軟骨組織形態(tài)學(xué)評(píng)分差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.591)。③小鼠腰椎終板軟骨組織中MMP-13和Col2α1表達(dá)水平測(cè)定結(jié)果。空白對(duì)照組和補(bǔ)腎活血方組小鼠腰椎終板軟骨組織中MMP-13陽(yáng)性細(xì)胞數(shù)占比均低于模型組(P=0.002,P=0.010),Col2α1陽(yáng)性表達(dá)面積比均高于模型組(P=0.000,P=0.026)。空白對(duì)照組與補(bǔ)腎活血方組小鼠腰椎終板軟骨組織中MMP-13陽(yáng)性細(xì)胞數(shù)占比的差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.226)。空白對(duì)照組小鼠腰椎終板軟骨組織中Col2α1陽(yáng)性表達(dá)面積比高于補(bǔ)腎活血方組(P=0.000)。④小鼠腰椎終板軟骨組織中pSmad2表達(dá)水平測(cè)定結(jié)果。空白對(duì)照組小鼠腰椎終板軟骨組織中可見(jiàn)大量pSmad2陽(yáng)性細(xì)胞。空白對(duì)照組和補(bǔ)腎活血方組小鼠腰椎終板軟骨組織中pSmad2陽(yáng)性細(xì)胞數(shù)占比均高于模型組(P=0.014,P=0.040)。空白對(duì)照組與補(bǔ)腎活血方組小鼠腰椎終板軟骨組織中pSmad2陽(yáng)性細(xì)胞數(shù)占比的差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.444)。結(jié)論:補(bǔ)腎活血方可延緩小鼠腰椎間盤(pán)退變的病理進(jìn)程,其作用機(jī)制可能與補(bǔ)腎活血方能夠通過(guò)促進(jìn)腰椎終板軟骨細(xì)胞中Smad2的磷酸化,下調(diào)MMP-13的表達(dá)并上調(diào)Col2α1的表達(dá)有關(guān)。
Abstract:
Objective:To observe the effects of Bushen Huoxue Fang(補(bǔ)腎活血方,BSHXF)on lumbar endplate cartilage in mice with intervertebral disc degeneration(IVDD),and to explore its underlying mechanism.Methods:Eighteen 10-week-old SPF-grade male C57BL/6J mice were randomized into blank control group,model group,and BSHXF group,6 cases in each group.All mice but the ones in blank control group were modeled by lumbar spine instability(LSI)surgery for inducing IVDD.The next day after successful modeling,the mice in BSHXF group were intervened by intragastric administration with BSHXF extract,while the ones in blank control group and model group with the same dose of normal saline,once a day for consecutive 8 weeks.After the end of the last intervention,all mice were sacrificed by cervical dislocation,and their L4 and L5 vertebrae were harvested for Micro-CT examination,and the relative height of the L4-5 intervertebral disc was calculated.Furthermore,the pathological changes of the lumbar endplate cartilage tissues were observed,and the morphological score of lumbar endplate cartilage tissues was calculated,meanwhile,the expression levels of collagen typeⅡalpha 1 chain(Col2α1),matrix metalloproteinase-13(MMP-13),and pSmad2 in the lumbar endplate cartilage tissues were detected.Results:①The Micro-CT analysis results of the lumbar vertebrae.The blank control group and BSHXF group presented lower bone volume fraction(BVF)and fewer trabecular number(Tb.N)in the ossification area of lumbar endplate cartilage,and higher relative height of intervertebral disc compared to model group(P=0.000,P=0.022; P=0.002,P=0.001; P=0.000,P=0.000).Moreover,the BSHXF group exhibited higher BVF compared to blank control group(P=0.003),while,there was no significant differences between the 2 groups in the Tb.N and relative height of intervertebral disc(P=0.882,P=0.794).②The pathological observation of the lumbar endplate cartilage tissues.In blank control group,the lumbar endplate chondrocytes presented as low heterogeneity,normal cartilage thickness,and a small ossified area.Compared with that of blank control group,the changes in the lumbar endplate chondrocytes,manifesting as significantly increased heterogeneity,thinned cartilage layer,and an enlarged ossified area,were observed in mice of model group; while,compared with that of model group,the changes in the lumbar endplate chondrocytes,manifesting as reduced heterogeneity,increased cartilage thickness,and a decreased ossified area,were observed in mice of BSHXF group,but all these did not reach the levels of blank control group.Besides,the morphological score of lumbar endplate cartilage tissues was lower in blank control group and BSHXF group compared to model group(P=0.000,P=0.000),with no significant difference between blank control group and BSHXF group(P=0.591).③The expression levels of MMP-13 and Col2α1 in the lumbar endplate cartilage tissues.The proportion of MMP-13-positive cells was lower,while the ratio of Col2α1-positive expression area was higher in blank control group and BSHXF group compared to model group(P=0.002,P=0.010; P=0.000,P=0.026).Furthermore,the ratio of Col2α1-positive expression area was higher in blank control group compared to BSHXF group(P=0.000),while there was no significant difference in the proportion of MMP-13-positive cells between the 2 groups(P=0.226).④The expression level of pSmad2 in the lumbar endplate cartilage tissues.A high percentage of pSmad2-positive cells were observed in the lumbar endplate cartilage tissues of blank control group.The proportion of pSmad2-positive cells in the lumbar endplate cartilage tissues was higher in blank control group and BSHXF group compared to model group(P=0.014,P=0.040),with no significant difference between blank control group and BSHXF group(P=0.444).Conclusion:BSHXF can delay the pathological progression of lumbar IVDD in mice.It may work by down-regulating the expression of MMP-13,and up-regulating the expression of Col2α1 through promoting the phosphorylation of Smad2 in lumbar endplate chondrocytes.

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備注/Memo

備注/Memo:
基金項(xiàng)目:浙江省自然科學(xué)基金探索項(xiàng)目(LY23H270005)
通訊作者:王萍兒 E-mail:[email protected]
更新日期/Last Update: 1900-01-01